Histochemical analysis of the digestive tract glycoconjugates

在过去的两年里，我的课题组研究了以下四个项目。(1)以大鼠胃和结肠为模型器官，确定了标记凝集素的最佳染色条件。本研究采用包埋后染色法对胶体金标记的凝集素和亲水树脂进行联合染色。在众多课题组中，我们课题组属于实验中使用多种凝集素的课题组。(2)采用上述染色条件，对大鼠腋腺各细胞类型进行染色。我们观察到从黏液颈细胞到主细胞的标记强度降低。主细胞的分泌颗粒几乎没有标记。我们还发现了一个介于黏液颈细胞和主细胞之间的中间细胞。由于胃蛋白酶原1也存在于这三种细胞中，因此我们认为黏液颈细胞是主细胞的前体细胞。(3)在结肠黏液细胞中研究了细胞的末端糖基化位点。HPA、DBA、SBA染色高尔基体顺侧，RCA-1和PNA染色高尔基体反侧，UEA-1和LFA染色高尔基体最顺侧。电镜放射自显影所证实的事实又被凝集素细胞化学所证实。(4)利用GSA-11凝集素观察该凝集素在大鼠基底腺的发育染色变化。这种凝集素抑制了胚胎的覆盖上皮和腺体基部。出生后，这种凝集素染色基部到中间区域。这种分泌物在成人颈部粘液区染色。在基底腺发育过程中，染色转移已被确定。

During past two years, the following four projects have been studied in my research group.(1) Using rat stomach and colon as model organ, the optimal staining conditions of labeled lectins have been established. In our study, postembedding staining method was used in combination to the colloidal gold labeled lectins and hydrophilic resin. Among many research groups, our group belongs the one which uses many kinds of lectins in the experiment.(2) Using above-mentioned staining conditions, we stained the various cell types of the rat hundic gland. We observed the decrease of the labeling intensity from mucous neck cell to chief cell. The secretory granules of the chief cell were hardly labeled. we also found an intermediate cell belonging between mucous neck cell and chief cell. Since pepsinogen 1 is also found in these three types of cells, we concluded the mucous neck cell is the precursor cell of the chief cell.(3) Terminal glycosylation sites of the cell was studied in the colonic mucous cell. HPA, DBA, SBA stained the cis side of the Golgi apparatus, RCA-1 and PNA stained the trans side of the Golgi and UEA-1 and LFA statined the transmost elements of the Golgi apparatus. The facts which had been ascertained by the electron microscopic radioautography have been reconfirmed by the lectin cytochemistry.(4) Using GSA-11 agglutin, we observed the developmental staining changes of this lectin in the fundic gland. This lectin statined the covering epithelia and gland base at the embryo. After birth, this lectin stained basal to intermediate regions. This lection stains the mucous neck region at adult. The staining shift has been ascerteined in this lection during the development of the fundic gland.