Pathological study on amyloidosis using of monoclonal antibodies against the synthesized peptides.

O.抗AA蛋白的单克隆抗体KM-268是针对人工合成的人淀粉样蛋白（AA）氨基酸序列短至37-47位的氨基酸序列而制备的。对牛、小鼠、天鹅和人AA的淀粉样组织进行T免疫组化染色，可用于不同种属AA型淀粉样变性的鉴别。以小鼠血清淀粉样蛋白A（SAA）氨基酸序列为抗原，合成肽段，制备了抗SAA蛋白A单克隆抗体（KM-503）。免疫组化法检测表明，AA对小鼠肝细胞内SAA蛋白有抑制作用，但对各器官内淀粉样蛋白沉积无抑制作用。阳性免疫反应的肾小管。这一发现表明SAA通过尿液过滤，并在肾小管上皮细胞中重吸收，而miIII。淀粉样蛋白增强因子（AEF）a）衰老小鼠中的AEF;在脾匀浆和9周龄小鼠中测试AEF活性。在52周龄小鼠的培养上清中存在较强的AEF活性。在匀浆小鼠中仅观察到弱活性，并且没有来自9周龄小鼠的活性。提示AEF随年龄增长而增加。B）中性粒细胞中AEF的免疫细胞化学证据; AEF是由淀粉样蛋白生成刺激引起的。制备的抗AEF抗血清与受刺激小鼠脏器中的中性粒细胞反应强，与正常小鼠脏器中的中性粒细胞反应弱。结果提示，AEF可能与中性粒细胞有关，且中性粒细胞AEF活性随年龄增长而增强

O. Monoclonal antibody against AA protein A monoclonal antibody (KM-268) was raised against the synthesized shor to 37-47 residues in amino acid sequence of human amyloid A (AA) protein. T immunohistchemically to amyloid tissues from cow, mouse, swan and human AA was useful for the identification of AA type amyloidosis of various speciesII. Monoclonal antibody against SAA protein A monoclonal antibody (KM-503) was raised the synthesized peptides cor residues in amino acid sequence of murine serum amyloid A (SAA) protein. Thimmunohistochemically to SAA protein in the hepatocytes of mice after an am but not to the amyloid deposits in various organs. Positive immunoreaction the uriniferous tubles. The finding suggests that SAA is filterered through urine and is reabsorbed in the epithelium of the uriniferous tubule, and miIII. amyloid enhancing factor (AEF) a) AEF in aging mice; AEF activity was tested in spleen homogenates f and 9-week-old mice. Strong AEF activity was present in the supernatant of from the 52-week-old mice. Only weak activity was observed in the homogenat mice, and none was from the 9-week-old mice. The findings suggest that AEF with aging. b) An immunocytochemical evidence of AEF in neutrophils; AEF was extr recieved an amyloidogenic stimulation. An antiserum against AEF was raised antiserum reacted strongly with the neutrophils in the organs of mice with stimulation, and weakly in normal mice. The findings suggest that AEF might neutrophils, and that AEF activity in the neutrophils increases with the am