Effect of Fibronectin or Laminin on Liver Regeneration following Hepatectomy.

1)研究了肝硬化（LC）患者和非肝硬化（LC）大鼠肝切除术后肝残体中纤维连接蛋白（FN）水平与再生过程的关系。人和大鼠肝切除术后血浆FN水平立即降低。患者和肝脏正常的大鼠分别在1个月和1周内恢复到术前血浆FN水平，但LC患者血浆FN低水平持续时间更长。FN水平与肝再生程度密切相关。这些结果表明血浆FN水平作为肝再生的一个参数是有用的。2)此外，我们还研究了添加FN或抑蛋白蛋白对大鼠肝切除术后肝脏再生过程的影响。大鼠肝切除70%后，立即腹腔给予纯化的人血浆FN和/或抑肽蛋白。同时给予FN和抑酶蛋白可显著提高^ 3h -胸苷的摄取、网状内皮系统的吞噬指数、有丝分裂指数和肝切除术后肝再生率，但单独注射抑酶蛋白对这些指标无显著促进作用。另一方面，补充吲哚美辛促进肝再生，在肝切除术后24小时显著抑制了氚化胸苷的摄取，克服了FN和抑肽蛋白对DNA合成的刺激作用。这些结果表明，FN可能是网状内皮系统的激活剂，特别是对Kupffer细胞的激活作用，Kupffer细胞在肝脏再生过程中刺激前列腺素的释放，促进DNA的合成，并且抑肽蛋白可能通过抑制FN的蛋白水解来增强FN的作用。3)硫乙酰胺所致肝硬化大鼠肝切除术后24小时，补充FN和抑肽蛋白也能增加DNA合成，但低于正常肝大鼠。

1) The relationship between plasm fibronectin (FN) levels and the regenerative process in liver remnants after hepatectomy was studied in patients and in rats with or without liver cirrhosis (LC). Plasma FN levels were reduced immediately after hepatectomy in human and rat. Both patients and rats with normal livers returned to the preoperative plasma FN livels within one month and one week, respectively, but low levels of plasma FN persisted longer in those with LC. FN levels were well correlated with the degree of the hepatic regeneration. These results suggest the usefulness of plasma FN levels as a parameter for liver regeneration. 2) Moreover, we studied the role of FN or aprotinin supplementation on the process of liver regeneration following hepatectomy in rats. After 70% hepatectomy, rats were immediately given purified human plasma FN and/or aprotinin intraperitoneally. The admi nistration of FN and aprotinin significantly increased ^3H-thymidine uptake, the phagocytic index of the reticuloendothelial system, the mitotic index, and the liver regeneration rate following hepatectomy, but the injection of aprotinin alone did not significantly promote those indices. On the other hand, the supplementation of indomethacin for the liver regeneration resulted in significant suppression of the tritiated thymidine uptake, 24 hours after hepatectomy, and overcame the stimulative effect of FN and aprotinin on DNA synthesis. These results suggest that FN may act as an activator of the reticuloendothelial system, and especially on the activation of Kupffer cells, which stimulate the release of prostaglandins promoting DNA synthesis in the process of liver regeneration, and, also aprotinin may enhance the effect of FN by inhibiting the proteolysis of FN. 3) In the liver cirrhosis rats induced by thioacetamide, the supplementation of FN and aprotinin also increased DNA synthesis, 24 hours after hepatectomy, but lower than those in normal liver rats.