Experimental study for the differntial conduction blockade of pain-mediating nerve fibers.

本研究的目的是探讨局麻药二布卡因和镇痛药Neurotropin对疼痛介导神经纤维的传导阻滞差异。观察各药剂不同浓度溶液孵育期间兔颈迷走神经诱发复合动作电位振幅的变化。3*10^<-3>%和3*10^<-2>%的西布卡因溶液分别对β和C组分造成不可逆的传导阻断。神经妥乐平没有引起a β和C组分的传导阻滞。这些结果表明，二布卡因和神经妥乐平都没有选择性阻断疼痛介导神经纤维的作用。虽然这些结果是我们在本研究中最初尝试的反向观察，但本研究中使用的方法被认为对体外研究神经纤维的差异传导阻滞是有用的。我们用这种方法研究了维生素B12和对羟基苯甲酸甲酯的作用。

The purpose of this study is to investigate the differential conduction blockade of pain-mediating nerve fibers by dibucaine, a kind of local anesthetics, and Neurotropin, an analgesic.Changes of the amplitudes of evoked compound action potentials elicited from excised rabbit cervical vagus nerve during incubation in solutions with various concentrations of each agent were observed.3*10^<-3>% and 3*10^<-2>% of sibucaine solution caused irreversible conduction blockade of abeta and C component respectively.Neurotropin did not cause any conduction blockade of both a beta and C components.These result indicate that neither dibucaine nor Neurotropin have an action to block pain-mediating nerve fibers selectively. Although these results were reverse observation which we attempted initially on this study, the method used on this study is thought tobe useful to investigate differential conduction blockade of nerve fibers in vitro.We are investigating the effect of Vitamin B12 and p-hydroxybenzoic acid methyl ester with this method.