An Experimental pathological study on the functional Role of T Lymphocytes for the appearance and progression of lesions in oral cavity.

本研究采用MRL/Mp-lpr/lpr （MRL/l）小鼠，分别于出生后6、8、12、20和24周以上处死，免疫组化检查颌下腺、舌下腺、舌、颌下腺淋巴结、肺、肾、脾等组织病变。检测了部分MRL/l小鼠血清中的自身抗体。不同Lyt表型的外周血t细胞亚群检测主要采用抗小鼠单克隆抗体亲和素-生物素过氧化物酶复合物法（becton discinson co. ltd.）。采用荧光抗体法检测血清中免疫复合物、c_3片段和抗核抗体。可溶性核抗原检测采用单克隆法。mrl/l小鼠口腔组织紊乱是由lpr基因调控的遗传决定的辅助性T淋巴细胞（Thy-1, 2^+, Lyt-1^+）增殖引起的免疫复合物疾病的症状。结果表明，颌下腺肉芽肿性脉管炎的发生和发展过程中，早期出现的淋巴细胞局灶性浸润（Lyt-1^+）引起的多克隆B细胞活化和自身抗体产生引起的免疫复合物沉积起重要作用。同时发现免疫复合物沉积和巨噬细胞功能异常是舌部肉芽肿性脉管炎的重要原因。

In this study, MRL/Mp-lpr/lpr (MRL/l) mice were used and killed at 6, 8, 12, 20 and more than 24 weeks after birth for immunohistochemical examinations of lesions in the submandibular gland, sublingual gland, tongue, submandibular lymph nodes, lung, kidney, and spleen et al. Auto-antibodies in the serum were investigated in some MRL/l mice. Examination of peripheral t cell subsets by different Lyt phenotypes was mainly performed by the avidin-biotin peroxidase complex method using anti-mouse monoclonal antibodies (becton discinson co. ltd.). the fluorescent antibody method was used to detect immune complex and c_3 fragment and antinuclear antibody in the serum. soluble nuclear antigen was detected by ouchte-rlony assay. the tissular disorder of the oral cavity in mrl/l mice was revealed to be a symptom of immune complex disease caused by the proliferation of genetically determined helper T lymphocytes (Thy-1, 2^+, Lyt-1^+), regulated by lpr genes. It was demonstrated that polyclonal B cell activation provoked by the focal infiltration of lymphocytes (Lyt-1^+) emerging in the parenchyma at the early stage, and deposition of immune complex caused by production of autoantibody plays an important part in the appearance and progression of granulomatous angiitis in the submandibular gland. At the same time, deposition of immune complex and abnormal macrophage function were found to be important causes of granulomatous angiitis manifestating in the tongue.