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Cell Analysis of Epithelial Cytokines Expressed in Oral Mucosal Squamous Epithelium

口腔粘膜鳞状上皮表达的上皮细胞因子的细胞分析

基本信息

批准号：
05671526
负责人：
WATANABE Yoshihisa
金额：
$ 1.34万
依托单位：
Kanagawa Dental College
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1993
资助国家：
日本
起止时间：
1993 至 1994
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05671526/
关键词：
human gingival cultured cell IL-1 epithelial cytokine laser cytometer peroxidase antibody technique flourescein antibody technique LPS periodontal disease 螢光抗体法 IL-1alpha IL-1beta

项目摘要

In this study, we clarified the expression of IL-1alpha and IL-1 beta in human gingival epithelium of the periodontal disease. At the same time, using the first cultured cells of human gingival tissue, we examined the possibility that individual differences in the mode of appearance of IL-1alpha and IL-1 beta in gingival epithelium might affect the difference in onset, advance and clinical behavior of the periodontal disease.By means of the ACAS570 Laser Cytometer, analysis was made of cultured epithelial cells obtained from the gingivae of 10 patients six to 21 years old with non-flammatory periodontal tissue. The appearance of IL-1alpha was observed in all the cases with the occurrence ratio calculated gingival epithelial cells were from 1.34% to 6.99%. After an addition of LPS,the number of cells in which IL-1alpha occurred increased in all the cases, 1.34 to 3.34 times the rate for the cultured cells without LPS.In one case (a 6-year-old male), the number of the cultured gingival e … More pithelial cells which manifested the occurrence of IL-1alpha increased by 23.31% with an addition of LPS,which was remarkable as compared with all therest. The appearance of IL-1 beta was also observed in the cultured epithelial cells from all of the 10 examines. The occurrence ratio was between 1.47% and 14.9%. When LPS was added, in five cases out of 10, the number of the cells exhibiting the existence of IL-1beta increased 1.43 - 2.8 times. On the other hand, hardly any increase in the occurrence of IL-1beta was noted in the other five cases including the case of the 6-year-old male, which demonstrated a remarkable increase in the occurrence of IL-1 alpha. To sum up, we can say that the number of gingival epithelial cells showing the existence of IL-1alpha and IL-1 beta differs from individual to individual, and increases in the number of the IL-1 alpha or IL-1beta occurring cells by LPS also vary depending on individuals. These findings suggest that the gingival epithelium, which has been considered to serve as a mechanical filtration or protective barrier, has an immunological function and that individual differences in immunocompetence have some implication in the occurrence, advanceand clinical behavior of periodontal disease. Less

在本研究中，我们明确了IL-1 α和IL-1 β在人类牙周病牙龈上皮中的表达。同时，利用首次培养的人类牙龈组织细胞，我们探讨了牙龈上皮中IL-1 α和IL-1 β出现方式的个体差异可能影响牙周病发病、进展和临床行为的差异。采用ACAS570激光细胞仪对10例6 ~ 21岁非炎症性牙周组织患者的牙龈上皮细胞进行了分析。所有病例均可见il -1 α的出现，计算牙龈上皮细胞的发生率为1.34% ~ 6.99%。添加LPS后，所有病例中出现il -1 α的细胞数量均增加，是不添加LPS培养细胞的1.34 ~ 3.34倍。其中1例（6岁男性）培养的牙龈上皮细胞中，表现出il -1 α发生的上皮细胞数量增加了23.31%，与其他对照组相比，增加了23.31%。IL-1 β的出现也在所有10个检查的培养上皮细胞中观察到。发生率在1.47% ~ 14.9%之间。添加LPS后，10例中有5例显示存在il -1 β的细胞数量增加了1.43 ~ 2.8倍。另一方面，包括6岁男性病例在内的其他5例病例中，IL-1 β的发生率几乎没有增加，这表明IL-1 α的发生率显著增加。综上所述，我们可以说，显示IL-1 α和IL-1 β存在的牙龈上皮细胞的数量因人而异，LPS增加IL-1 α或IL-1 β发生的细胞的数量也因人而异。这些结果提示，一直被认为具有机械过滤或保护屏障作用的牙龈上皮具有免疫功能，个体免疫能力的差异与牙周病的发生、进展和临床行为有一定的关系。少