The effct of Pulsed electromagnetic fields on proliferation and differentiation of rabbit costal chondrocytes in culture

已有研究表明，脉冲电磁场(PEMF)刺激可增强原代培养兔肋软骨细胞甲状旁腺激素(PTH)对环磷酸腺苷(CAMP)、鸟氨酸脱羧酶和氨基葡萄糖多糖合成的刺激作用，提示脉冲电磁场刺激增强了培养软骨细胞对PTH的敏感性，促进了软骨细胞的分化。但PEMF对软骨细胞增殖无明显影响。PEMF刺激仅在甲状旁腺功能亢进时起作用。在软骨衍生因子、成纤维细胞生长因子、P物质和胰岛素样生长因子存在的情况下，它没有任何作用。用正常大鼠肾细胞研究了脉冲电场(PEF)刺激对细胞增殖的影响。在0.3%胎牛血清存在时，PEF刺激使~lt；d13@&gt；d3H-胸腺嘧啶核苷掺入DNA增加了30%。进一步增加FCS浓度可加速细胞增殖，减弱PET刺激的作用。此外，PEF和PEMF刺激还会影响软骨细胞颗粒培养过程中碱性磷酸酶的活性。

Previous studies have shown that pulsed electromagnetic field (PEMF) stimulation potentiates parathyroid hormone(PTH) stimulations of cAMP, ornithine decarboxylase and glucosaminoglycan syntheses in primary cultures of rabbit costal chondrocytes.This indicates that PEMF stimulation angments the sensitivity of cultured cartilage cells to PTH and promotes their differentiation. However, PEMF had little effect on chondrocyte proliferation. The effect of PEMF stimulation was observed only in PTH. It did not give any effects in the presence of cartilage derived factor, fibroblast growth factor, substance-P, and insulin-like growth factor. In the present study, the effect of pulsed electric field(PEF) stimulation on cell proliferation was study using Normal rat kidney cells. PEF stimulation caused 30% increase in the incorporation of <@D13@>D3H-thymidine into DNA in the presence of 0.3% FCS. Further increase in the concentration of FCS accelerated the proliferation of cells and diminished the effect of the PET stimulation. Furthermore the PEF and PEMF stimulations affected alkaline phosphatase activity during the process of calcification in pellet cultures of chondrocytes.