Immunohistochemical investigation on the effect of the bone resorbint factors

用免疫组织化学方法研究了白细胞介素-1 β在人和大鼠根尖周围病变中的定位。人类根尖切除后的根尖周围病变用PLP固定剂固定5小时。这些典型部位放置在4%的PFA中，并在10%的EDTA溶液中脱钙10天。为了免疫组织化学染色il -1 β，我们采用了亲和素标记的生物素方法，使用过氧化物酶和碱性磷酸酶。在这种方法中，切片与抗体（抗人重组il -1 β小鼠单克隆抗体）室温孵育2小时。发现几种细胞类型对il -1 β表现出阳性免疫反应性。白细胞介素-1 β染色特别深的细胞类型有中性粒细胞、单核细胞、内皮细胞和破骨细胞。总之，这项研究表明白细胞介素存在于浸润到根尖周围病变的细胞中。因此，这种细胞因子可能参与了牙槽骨的吸收。

The localization of the cytokine interleukin-1beta was studied immunohistochemically im human and rat periapical lesions.Human periapical lesions obtained by the apicoectomy were fixed with PLP fixative for 5 hours. Tat perispical sites were placed in 4% PFA and decalcified in 10% EDTA solution for 10 days.For the purpose of staining immunohistochemically for IL-1beta, we followed an avidin labelled biotin method with the use of peroxidase and alkaline phosphatase. In this mothod, sections were incubated for 2 hours, at room temperature, with the antibody (anti IL-1beta mouse monoclonal antibody against human recombinant IL-1beta).Several cell types were found to display positive immunoreactivity for IL-1beta. Cell types which stained particularly dark for UL-1beta were neutrophils, mononucleated cells, endothelial cells and osteoclasts.In conclusion, this investigation has demonstrated that interleukin is present in cells infiltrating into periapical lesions. Hence, this cytokine may be involved in the resorption of alveolar bone.