Induction of dentin-bridge formation after pulpotomy by biomaterials

牙本质非胶原基质在牙本质矿化过程中起着重要作用。本研究旨在探讨非胶原牙本质基质能否在实验动物皮下组织中诱导硬组织形成。通过组织化学或免疫组织化学方法检测牙本质磷蛋白和_2HS糖蛋白的组织分布，发现两者均存在于牙本质中，与矿物晶体密切相关。这些数据表明，这些蛋白质可能在矿化过程中发挥作用。然后，通过将这些蛋白质皮下植入大鼠体内，试图诱导硬组织的形成。将含牙本质磷蛋白的聚丙烯酰胺凝胶盘植入大鼠皮下，凝胶盘内形成矿物晶体。这些结果表明，在牙髓切断术后，将磷酸盐放置在牙髓表面，可能会诱导牙本质桥的形成。

Non-collagenous matrix of dentin has been thought to play important role in the process of dentin mineralization. In this study, we determined whether non-collagenous dentin matrix can induce hard tissue formation in the subcutaneous tissue of experimental animals.When tissue distributions of dentin phosphophoryn and _2HS glycoprotein were examined by histochemical or immunohistochemical method, it was found that both of them were present in dentin being strongly associated with mineral crystals. The data suggested that these proteins might play role in the process of mineralization. Then, induction of hard tissue formation was attempted by subcutaneous implantation of these proteins into rats. When polyacrylamide gel disc containing dentin phosphophoryn were subcutaneously implanted into rat, mineral crystals were fromed in the gel disc. These results suggest that phosphophoryn may be able to induce the formation of dentin-bridge when it is placed on the surface of dental pulp after pulpotomy.