Analysis of factors relevant to the therapies for immunologically mediated skin diseases.

1.利用具有或不具有亲表皮性的小鼠克隆T细胞系，研究了某些T细胞优先迁移到表皮的机制。GM-CSF受体在所有受试的亲表T细胞克隆的表面都有表达，但其表达并不局限于亲表T细胞克隆。LFA-1主要表达于亲表T细胞克隆表面，而非亲表T细胞克隆除1例外均为阴性或极弱阳性。结论：1.GM-CSF受体的表达使T细胞在角质形成细胞来源的GM-CSF的刺激下增殖，高水平的LFA-1表达使T细胞与表皮黏附，这可能是嗜表T细胞的一种结构性特征。皮内注射亲表皮型T细胞克隆引起的皮肤移植物抗宿主病后，自发恢复的小鼠对随后T细胞诱导皮肤移植物抗宿主病的尝试产生抵抗力。随着表皮结构的破坏，Thy 1^+表皮细胞的数量急剧增加，这对于保护表皮完整性免受T细胞的额外攻击是很重要的。

1. Mechanism by which certain T cells preferentially migrate into the epidermis was investigated by using murine cloned T-cell lines with or without epidermotropic nature. Expression of GM-CSF receptor was observed on the surface of all epidermotropic T-cell clones tested, although the expression was not restricted to the epidermotropic T-cell clones. LFA-1 was preferentially expressed on the surface of epidermotropic T-cell clones, while non-epidermotropic T-cell clones were negative or very weakly positive for LFA-1 with one exception. Expression of GM-CSF receptor that enables T cells to proliferate in response to keratinocyte-derived GM-CSF and high levels of LFA-1 expression that allows T cells to adhere to epidermis may be a constitutive property of epidermotropic T cells.2. Mice that had spontaneously recovered from the cutaneous GVHD induced by the intradermal injection of epidermotropic T-cell clones became resistant to subsequent attempts to induce the cutaneous GVHD by the T cells. The great increase in the number of Thy 1^+ epidermal cells following destruction of epidermal structures is important in protecting the epidermal integrity from an additional attack by T cells.