Mechanisms by which fucosyltransferases regulate epidermotropic migration of T cels

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In this study, we for the first time developed a new mAb specific for Fuc-TVII that provides a means for the reliable identification of the enzyme in preparations of mixed cell populations or in tissues by immunohistochemistry. Using this mAb to Fuc-TVII, we found that in peripheral blood mononuclear cells (PBMC) and the purified CD4^+ and CD8^+ populations, unlike cell lines, the expression of Fuc-TVII in the cytoplasm was not necessarily associated with the surface expression of the CLA epitope : some cells expressed high levels of surface CLA but failed to express cytoplasmic Fuc-TVII and vice versa. Nevertheless, the percentages of Fuc-TVII-expressing CD4^+ or CD8^+ cells were close but not identical to those of CLA-expressing cells. According to the expression pattern of CLA and Fuc-TVII, the CD4^+ and CD8^+T cells consisted of three populations : Fuc-TVII^+CLA^+, Fuc-TVII^-CLA^+, and Fuc-TVII^+CLA^-. Our kinetics studies after in vitro stimulation with anti-CD3 mAb showed that the Fuc-TVII^-CLA^+ subset represents early activated cells that eventually differentiate into the Fuc-TVII^+CLA^+ subset and subsequently the Fuc-TVII^-CLA^+ subset. The capability of CD4^+ T cells to bind E-selectin was detected in the Fuc-TVII^+CLA^- and Fuc-TVII^+CLA^+ subset, but not the Fuc-TVII^- CLA^+. The Fuc-TVII^- CLA^+ subset was observed in increased number in PBMC coincident with resolution of the immune activation in various inflammatory settings.