Localization and Alteration of Non Histon Proteins and Nucleoli Antigen in Normal Squamous Epithelium and Squamous Cell Carcinoma

用石蜡切片免疫分析和免疫沉淀法对人舌源性克隆性鳞状细胞癌细胞系的染色体和核仁组分制备的非希斯顿蛋白-DNA复合物进行了表征。存在于人鳞状细胞癌细胞系中的120 kD核抗原和60 kD核仁抗原是从染色质和核仁中提取的碱性蛋白。在NA细胞、人角化鳞状细胞癌和非角化鳞状细胞癌中观察到这些抗体与染色质和核仁的DAB反应。用这些抗体检测正常鳞状上皮、白斑、上皮不典型增生和乳头状瘤，均未发现DAB反应。但在少数上皮异型增生细胞中发现DAB反应呈弱阳性，这些细胞具有恶变的细胞亚群和细胞单态性，这些区域可能已经发生了局灶性恶变，这种抗原可能是阶段性或组织特异性的。胎儿组织中抗原的检测将影响这一发现在恶性肿瘤疾病临床诊断中的潜在价值。

Non histon protein-DNA complexes prepared to chromachin and nucleoli fractions from a human tongue-derived clonal squamous cell carcinoma cell line was characterized with immuno-analysis and immunoprecipitation by paraffin sections. The 120 kD nuclear antigen and 60 kD nucleoli antigen present in the human squamous cell carcinoma cell line is a basic protein that is extracted from chromatin and nucleoli. DAB reaction of these antibodies to chromatin and nucleoli was observed in NA cells, human keratinized squamous cell carcinoma and non-keratinized squamous cell carcinoma. With these antibodies, DAB reaction was not found in normal squamous epithelium, leukoplakia, epithelial dysplasia and papilloma. But, slightly positive DAB reaction was found in a few cells of epithelial dysplasia which have cell atypia and cell ploemorphism for malignant changes ; these area may have been undergoing focal malignant changes.Such an antigen might be stage or tissue-specific. Detection of the antigenin fetal tissue would influence the potential value of this finding in clinical diagnosis of malignant neoplastic disease.