Physico-chemical Basis of Energetics and Dynamics of Membtane Proteins

由于本学期是这个项目的最后一年，我们集中讨论了一些重要的具体主题，这些主题是由成员们所取得的进展或引起人们极大兴趣的。之后，我们进行了全面的讨论。具体课题的题目是L。从与色觉相关的光感受器蛋白的一级结构中估计的视觉色素的系统发育树。2.光诱导分子内电子转移的飞秒和皮秒动力学。3.傅立叶变换红外光谱的原理及应用。4.蛋白质中的电子转移路径：Kuki和Wolyness的量子力学蒙特卡罗模拟和Berrarn理论的评述，Berrarn理论是通过通键机制的电子隧穿基元。5.用量热法观察蛋白质的热力学。6.光吸收光谱傅里叶变换激发态动力学。在综合讨论中，我们对本项目的活动情况进行了调查。L：成员…的专一性更多的ANE蛋白：我们参照球状蛋白的已知性质进行了比较研究。我们对蛋白质的多次折叠螺旋结构的稳定性和功能进行了研究。我们考察了膜蛋白中电离氨基酸残留物的热力学稳定性和反应活性。2.能量学：我们考虑了能量学如何对蛋白质结构的松弛起到调节作用。我们从能量学的角度研究了这种高效的能量转换和特定的反应机理。3.动力学：我们应用飞秒和皮秒光谱研究了视网膜蛋白质的光异构化动力学。。我们还发展了一种新的光吸收光谱傅里叶变换技术，用于研究激发态动力学。在这些讨论的基础上，我们都深刻地认识到，从能量学和动力学的角度对膜蛋白的结构和功能进行光化学研究，作为广泛的生命科学的一个基础领域，正变得比以前更加重要。较少

since this term is the last year of this project, we focused our discussion on some important specific topics which were progressed by the members or attracted much interests. After them, we made a comprehensive discussions. The title of the specific topics are l. Phylogenetic tree of visual pigments as estimated from the primary structure of photoreceptor proteins related to color vision. 2. Femto-and picoseconds dynamics of photoinduced intramolecular electron transfer. 3. Principle and application of FTIR spectra. 4. Path of electron transfer in the protein madia : Reviews of Quantum-mechanical Monte Carlo simulation by Kuki and Wolyness and of Berrarn theory the electron tunneling matrix element by the throughbond mechanism. 5. Thermodynamics of proteins as observed by the calorimetry. 6. Excited state dynamics by the Fourier transform of the optical absorption spectrum. In the comprehensive discussions, we made survey of our activity of the present project. l. Specificity of membr … More ane proteins : We made comparative study with reference to the well-known properties of the globular proteins. We proceeded our study on the stability and function of the several times folded helical structure of proteins. We investigated the thermodynamical stability and reactivity of ionized amino icid resdues inside the membrane proteins. 2. Energetics : We considered how the energetics plays a role of regulation the relaxation of protein structures. We investigated the highly efficient energy conversion and specific reaction mechanisms from the viewpoint of the energetics. 3. Dynamics : We applied the femto-and picosecond spectroscopy to the photoisomerization dynamics of retinal proteins. . We also developed a new technique of Fourier tranform of optical absorption spectrum for the study of excited state dynamics. Based on these discussions, we all deeply recognized that the phosico-chemical study of the structure and function of membrane proteins from the standpoint of energetics and dynamics is becoming much more important than before as a basic area of the broad life science. Less