Polymorphism of oligosaccharide structure in glycoconjugaates

调查人员分为以下3组。第1组和第2组的调查人员得到了第3组获得的信息的支持。第1组：肿瘤细胞或生殖细胞碳水化合物抗原分析及单克隆抗体纯化。Kannagi R.研究了表达唾液酰基或唾液酰聚焦基1型链聚乳胺的癌症相关血清糖蛋白抗原的定量和定性特征。Yamaguchi T.分析了人胃癌的抗原。Imai K.研究了碳水化合物链单克隆抗体对人肝细胞癌相关抗原抑制裸鼠肿瘤生长的作用。Tsuji Y.研究了人类精子碳水化合物抗原，该抗原由一种来自一名血清中含有抗精子抗体的不育妇女的抗精子人单克隆抗体定义。第2组：细胞表面糖基化多态性及其结构和功能。Muramatsu T.和Ozawa M.研究了从人胃癌细胞分离的花生凝集素受体碳水化合物部分的多态性。Hayashi K.研究了加利福尼亚鱼雷烟碱乙酰胆碱受体的寡糖多态性。Nakashima等研究了小鼠淋巴细胞和淋巴瘤细胞中寡糖的细胞类型和成熟阶段依赖性多态性。Kuroda Y.研究了PC-12嗜铬细胞瘤细胞中肿瘤相关岩藻糖脂苷的特征。第3组：建立简单、重现、灵敏的n -低聚糖结构测定方法。Takahashi N.研究了二维图谱技术分离和表征n -链低聚糖。Arata Y.积累了n -连接寡糖的^1H NMR测量数据。石原H.研究了糖蛋白中唾液酸的分析方法。

Investigators were classified into 3 groups as follows. Investigators belong to groups 1 and 2 were supported with the information obtained by group 3.Group 1 : Analyses of carbohydrate antigens in cancer cells or germ cells and purification of the monoclonal antibodies.Kannagi R. investigated quantitative and qualitative characterization of cancer associated serum glycoprotein antigens expressing sialosyl- or sialosyl-fucosyl type 1 chain polylactosamin.Yamaguchi T. analyzed antigens on human gastric carcinoma.Imai K. investigated a monoclonal antibody to carbohydrate chain on human hepatocellular carcinoma-associated antigen suppressed tumor growth in nude mice.Tsuji Y. investigated human sperm carbohydrate antigens defined by an antisperm human monoclonal antibody derived from an infertile woman bearing antisperm antibodies in her serum.Group 2 : Glycosylation polymorphism on the cell surface, its structure and function.Muramatsu T. and Ozawa M. investigated polymorphism of carbohydrate moieties of peanut agglutinin receptor isolated from human gastric cancer cells.Hayashi K. investigated oligosaccharide polymorphism of nicotinic acetylcholine receptor from Torpedo californica.Nakashima I. investigated cell type and maturation stage-dependent polymorphism of oligosaccharides on murine lymphocytes and lymphoma cells.Kuroda Y. investigated characterization of tumor associated fucogangliosides from PC-12 phenochromocytoma cells.Group 3 : Development of analytical method for simple, reproducible and sensitive determination of the structures of N-linked oligosaccharides.Takahashi N. investigated two-dimensional mapping technique for separation and characterization of N-linked oligosaccharides.Arata Y. accumulated the data of ^1H NMR measurements for N-linked oligosaccharides.Ishihara H. investigated analytical procedure for determination of sialic acids in glycoproteins.