Polypeptide Microshpheres

用我们独特的方法制备了聚γ -甲基l -谷氨酸的多孔球形颗粒。通过调节悬浮条件（如聚乙烯醇作为悬浮介质和聚γ -甲基谷氨酸）的浓度、搅拌速度和悬浮温度)来控制球的直径。得到了平均直径为0.1 ~ 1000 μ m的聚γ -甲基l -谷氨酸微球，球化过程中使用稀释剂实现了微球的宏观网状。具有部分极性基团的疏水溶剂，如十氢萘、邻苯二甲酸二酯和油酸，是制备大孔的有效溶剂。测定了球内肽链的二级结构。二级结构的含量可通过料液孵育和甲酸处理来调节。用柱法检测了球对蛋白质的亲和力。这些球体对蛋白质有特殊的亲和力。这与这里提出的结果一致，在PKC激活剂的存在下，转化发生率降低。

Porous and spherical particles of poly (gamma-methyl L-glutamate) were prepared by our unique method. The diameter of spheres was controlled by adjusting the suspension condition, for example the concentration of poly(vinyl alcohol) as a suspension medium and poly (gamma-methyl L-glutamate), stirring spped and suspension temperature. As a result, we obtained poly (gamma-methyl L-glutamate) spheres with average diameters of 0.1 mum to 1000 mum The macroreticulation of spheres was achieved by use of diluent in the process of sphering. Hydrophobic solvents with partial polar groups were effective to make macroporous, for example decahydronaphthalene, dialkylphthalate and oleic acid. The secondary structures of peptide chains in the spheres was determined. The content of secondary structures is adjustable by incubation of material solution and treatment of formic acid. The affinity of spheress for proteins were examined by a column method. The spheres have specific affinity for proteins. This is in agreement with the result presented here, where transformation incidences were reduced in the presence of PKC activators.