DNA Fingerprinting by Means of a Nonradioactive Probe for Individual Identification

使用ECL（增强化学发光）技术进行非放射性DNA指纹识别。用Hae III酶切的三到五杯基因组DNA在1.3%琼脂糖中电泳并转移到尼龙膜上。碱固定后，将印迹与过氧化物酶标记的微型卫星“myo”探针杂交并洗涤。在光敏膜上检测了酶标催化ECL反应产生的蓝光。采用市售试剂盒进行DNA检测和标记。另一个微型卫星探测器可以在不移除myo的情况下被谴责。该技术在DNA检测中具有与放射性方法相当的灵敏度，并且比使用磺化、半抗原标记和生物素标记DNA探针的其他非同位素方法更容易重新探测。该方法可应用于亲子鉴定、合子诊断和骨髓移植的确证。因此，由于冷探针的处理是安全且不受污染的，该技术被认为适用于涉及个人识别的日常工作。

A nonradioactive DNA fingerprinting using ECL (Enhanced Chemi-Luminesent) technique was performed. Three to five mug of genomic DNA digested with Hae III was electrophoresed in 1.3% agarose and transferred onto nylon membrane. Following alkali fixation, the blot was hybridized with peroxidase-labeledminisatellite " myo " probe and washed. Blue light araising from ECL reaction catalyzed by the enzyme label was detected on a light sensitive film. A commercially available kit was employed for DNA detection and labeling. Another minisatellite probe could be reprobed without removing myo. This technique had comparable sensitivity to radioactive method in DNA detection, and was far easier to reprobe than other non-isotopic methods using sulfonated, hapten-labeled and biotin-labeled DNA probe.Actually, this method could be applied to paternity testing, zygosity diagnosis and confirmation of bone marrow engraftment. Thus, since the handling of cold probe is safe and not contaminable, this technique is considered feasible for routine work involving individual identification.