ImmunoーElectronmicroscopy of the Formation and Resorption of the Basement Membrane in the Tooth Enamel Organ

本研究演示在大鼠和猴子牙釉质器官中进行了Laminin的超结构局部化，类型IV聚合物和酸磷酸酶（ACP酶）。用于Laminin和类型IV聚合物的免疫-被发现在底层搪瓷表皮和差异化的淀粉样蛋白的底层薄膜中。反应产品经常发生在整个内皮素细胞内的内离子体视网膜。在不同的淀粉样蛋白质中，等离子体-薄膜在距离末端导致的深度，不规则的入侵中消除。各种各样的数字可以容纳被感染的等离子体薄膜。反应产品在两种入侵和涂层坑中均不均匀。ACP酶的反应在分化的淀粉中被证明-细胞系蛋白颗粒在不同的淀粉中被证明。有时候，这些颗粒离被感染的等离子体膜很近。这些结果表明，基础膜是由内部搪瓷表皮细胞产生的，并由不同的淀粉样物质吸收的，强烈的拉明in反应发生在内部和外部的基础膜之间。Laminin反应产品were Observed within the rough endoplasmic reticulum of junctional epithelium cells and in minute invaginations formed at their distal等离子体膜adjacent to the basal lamina。小球体在感染中出现，也积极地对拉明反应。没有类型-IV聚合反应产品在室内的基础上可见。外部基底lamina显示出对类型的反应（IV Collagen）这些结果表明，其内部基质层含有laminin，但没有类型-IV聚合物和那些junctional epithelium细胞合成的laminin。

This study demonstrates ultrastructural localization of laminin, typeーIV collagen and acid phosphatase (ACPase) in the rat and monkey tooth enamel organs.Immunostaining for laminin and type-IV collagen was observed in the basement membranes of the inner enamel epithelium and differentiating ameloblasts. Reaction products occurred frequently in the rough endoplasmic reticulum of inner enamel epithelium cells. In differentiating ameloblasts, plasmaーmembrane undulation in the distal end caused deep, irregular invaginations. Various numbers of coated pits accompanied the invaginated plasma membrane. Reaction products occurred in both invaginations and coated pits. Reaction of ACPase was demonstrated in distalーcytoplasmic granules in differentiating ameloblasts. Sometimes the granules were very close to the invaginated plasma membranes. These results indicate that the basement membrane is produced by inner enamel epithelium cells and absorbed by differentiating ameloblasts.Intense laminin reaction occurred in both the internal and the external basal lamina. Laminin reaction products were observed within the rough endoplasmic reticulum of junctional epithelium cells and in minute invaginations formed at their distal plasma membranes adjacent to the basal lamina. Small spherical bodies occurring in the invaginations also reacted positively to laminin. No typeーIV collagen reaction products were seen in the internal basal lamina. The external basal lamina showed reaction to typeーIV collagen. These results indicate that the internal basal lamina contains laminin but no typeーIV collagen and that junctional epithelium cells synthesize laminin.