Defense Mechanisms in Eggs and Fry by Immunological Substances from Mother Fish.

在确认鱼卵中存在免疫球蛋白(IgM)后，进行了以下实验。建立了鲤鱼和虹鲑鱼的IgM检测体系。用纯化的IgM免疫兔。从抗血清中提纯免疫球蛋白，并进行生物化。结果：1.利用制备的免疫球蛋白和生物素标记的免疫球蛋白，建立了生物素-亲和素技术的酶免疫分析体系。使用EIA系统评估血清IgM水平，特别是性成熟。金鱼的免疫球蛋白M在春季产卵季节随水温升高而升高。另一方面，虹鱼在冬季产卵季节的免疫球蛋白M水平较低。对鲤鱼虫卵免疫球蛋白进行了分析。均质鸡蛋的上清液用高效液相色谱凝胶过滤进行分级。用SDS-PAGE分离各组分中的蛋白质，并转移到硝化纤维素膜上。用生物素化的抗IgM免疫球蛋白对膜进行免疫染色。含IgM组分的保留时间比血清IgM组分的保留时间长，表现出较低的相对分子质量。这种较低的相对分子质量是由较小的H链引起的。卵和鱼苗防御机制中分子差异的意义尚不清楚。

After confirming the existence of immunoglobulin (IgM) in fish eggs, following experiments were undertaken.1. Assay systems for IgM of carp and rainbow trout were made. Rabbit was immunized with purified IgM. From the antiserum, IgG was purified and was biotinized. Using the IgG and biotinized IgG, enzyme immunoassay (EIA) system by biotin-avidin technique was made.2. Serum IgM levels were estimated using EIA systems, with special reference to sexual maturation. In goldfish, IgM increased in the spring spawning season related with increase of water temperature. On the other hand, the IgM levels were rather low in the winter spawning season in rainbow trout.3. Egg IgM in carp was analyzed. The supernatant of homogenized eggs was fractionated by gel filtration using HPLC. Proteins in each fraction was separated by SDS-PAGE and transferred on nito-cellulose membrane. The membrane was immuno-stained using biotinized anti-IgM IgG. Retention time of fractions containing IgM was longer than that of serum IgM, showing lower molecular weight. This lower mw was caused by smaller H-chain. The significance of the molecular differences in the defense mechanisms of eggs and fry are unknown.