Monoclonal antibody immunohistochemistry of renewal pattern in olfactory receptor neurons

1. 研究了兔嗅球单克隆抗体在成年期、发育期间和嗅球切除后的嗅上皮中的免疫组织化学染色模式。一种单克隆抗体112D5 （MAb 2D5）染色成年兔的所有嗅觉受体细胞，而另一种单克隆抗体114G12 （MAb 4G12）染色受体细胞层上部的2/3至3/4。两种单克隆抗体均未染色支持细胞、基底细胞和鲍曼腺。MAb 2D5可在发育过程中染色嗅上皮和腭柄器官的所有受体神经元。4g12阳性细胞分散出现在胚胎第17天胎儿的上皮中。胚胎第25或26天，4g12阳性细胞位于浅表受体细胞层。4g12阳性和阴性受体神经元的排列是“浅阳性”和“深阴性”。此后，“表面阳性”细胞逐渐增加，而“深度阴性”细胞逐渐减少。这些变化一直持续到出生后30.5天。嗅受体神经元在球根切除后24小时出现轻微的减少。MAb 2D5染色所有受体神经元，包括变性神经元，但MAb 4G12免疫染色迅速下降，病变后7天内4G12阳性细胞消失，病变后4周重新出现6.4 g12阳性细胞。3个月时，4g12阳性细胞在受体细胞层的顶端排列成行，表明受体神经元的发育模式重现。6个月后，4g12阳性细胞逐渐增多，嗅上皮染色模式恢复。因此，MAb 4G12是第一个不特异于嗅觉神经元的标记物，可以用来表征成年哺乳动物嗅觉上皮变性和再生过程中的某些胚胎特征。少

1. Immunohistochemical staining patterns for monoclonal antibodies, produced against the rabbit olfactory bulb, were studied in the rabbit olfactory epithelium in adulthood, during development, and after olfactory bulbectomy.2. One monoclonal antibody 112D5 (MAb 2D5) stained all of the olfactory receptor cells in the adult rabbit, whereas the other 114G12 (MAb 4G12) stained the upper 2/3 to 3/4 of the receptor cell layer. Both MAbs did not stain the supporting cells, basal cells, and Bowman's glands.3. MAb 2D5 stained all of the receptor neurons in the olfactory epithelium and vomeronasel organ during development. 4G12-positive cells scatteringly appeared in the epithelium of the embryonic day 17 fetus. At embryonic day 25 or 26, 4G12-positive cells were situated in the superficial receptor cell layer.4. The arrangement in the 4G12-positive and -negative receptor neurons was 'superficial-positive' and 'deep-negative'. Thereafter, a gradual increase in the 'superficial-positive' cells w … More as accompanied by a decrease in the 'deep-negative' cells. These changes continued until postnatal day 30.5. A slight decrease in the olfactory receptor neurons occurred at 24 hours after the bulbectomy. MAb 2D5 stained all receptor neurons including degenerating neurons, but MAb 4G12 showed a rapid decrease in immuno-staining so that 4G12-positive cells disappeared within 7 days after lesion.6.4G12-positive cells reappeared at 4 weeks following lesion. By three months, 4G12-positive cells were arranged in a line at the apical region of the receptor cell layer, suggesting a recapitulation of developmental pattern of the receptor neurons. Thereafter, the 4G12-positive cells increased progressively and staining pattern of the olfactory epithelium recovered by 6 months.7. MAb 4G12 is thus the first marker, that is not specific to the olfactory neurons, and that can be used to characterize certain embryonic traits during the degeneration and regeneration of the olfactory epithelium in the adult mammal. Less