Structural Analysis of Flagella of Vibrio

有些弧菌在一个细胞上有两种鞭毛，极地单色鞭毛(M)和横向鞭毛(L)。以副溶血性弧菌鞭毛基因为探针进行Southern blot杂交，发现所有种弧菌的m -鞭毛基因和所有带l -鞭毛的弧菌的l -鞭毛基因DNA同源，而l -鞭毛探针与只带m -鞭毛的弧菌的杂交未观察到，说明这些弧菌中不存在l -鞭毛基因。两种不同大小的鞭毛蛋白分子，42 kd和44 kd，在所有被测弧菌的m鞭毛中发现。多克隆抗体凝胶扩散试验表明，这两种鞭毛蛋白具有免疫同质性。然而，单克隆抗体的结果表明，两种鞭毛蛋白中只存在一个表位。在同一物种的两个m鞭毛蛋白中发现了相似的n端氨基酸序列。然而，同一物种中L-鞭毛蛋白与m -鞭毛蛋白序列的相似性并不高，约为30 ~ 40%。这一水平与大肠杆菌等肠杆菌序列的相似性基本一致。L-鞭毛和M-鞭毛的基因似乎是独立进化的，尽管它们的祖先是共同的。Endentry，尽管它们的祖先应该是共同的。

Some vibrio species have two kinds of flagella, polar Monotrichous (M) and Lateral (L), on a cell. Southern blot hybridization with flagellar gene of Vibrio Pardhaemolyticus as the probe showed DNA homology of M-flagellar gene in all species of vebrios and L-flagellar gene in all L-flagella-having vibrios, whereas the hybridization L-flagellar probe with vibrios having only M-flagella was not observed, suggesting absence of L-flagellar gene in these vibrios. Two different-sized flagellin molecules, 42 kd and 44 kd, were found in M-flagella of all vibrios species tested. Gel diffusion test with polyclonal antibody showed immunological homogeneity of these two flagellins. However, result obtained by monoclonal antibodies suggested existence of epitopes which exist only in one of the two flagellins. Similar N-terminal amino acid sequences were found in the two M-flagellins of same species. However, similarity of the sequence between L- and M-flagellin of same species was not so high, approximately 30 to 40 %. This level was almost same with the similarity with the sequence of enterobacteria such as Salmonella Escherichia coli. The genes of L- and M- flagella seemed to evolve independently, although their ancestor be common. Endentry, although their ancestor should be common.