The effect afair pollutants on productibility of allergen specific antibody

为探讨空气污染物对吸入抗原致敏的影响，用豚鼠进行了2.5ppm的NO_2暴露实验。将60只雄性豚鼠随机分为3组，在500 L的卵清蛋白（OA）暴露室中暴露于25 mg/m3的OA气雾剂或5 mg/m3的OA或5 mg/m3的OA加2.5ppm的NO2，每天4小时，每周5天，连续4周。每隔一天、暴露前和暴露后4小时立即用振荡法测定呼吸流动阻力。暴露4周后，通过直接酶免疫测定法在血清和支气管肺泡液中测量针对OA的特异性抗体。暴露后第8- 10天出现第一次支气管反应。反应的强度取决于OA的浓度，并且与NO2暴露无关。血清和支气管肺泡液中OA特异性抗体与支气管反应强度的关系正在研究中。

In order to investigate th effect of air pollutants on allrgic sensitization to inhaled antigen, 2.5ppm N02 exposure experiments using guinea pigs were conductd. 60 male guinea pigs divided into three groups were exposed to 25mg/m3 ovalbumin (OA) aerosol or 5mg/m3 OA or 5mg/m3 OA plus 2.5ppm N02 in a original OA exposure system with a 500L exposure chamber for 4 hrs/day, 5days/week, for 4 weeks. Every other day, before and immediate after 4hr-exposure measurement of respiratory flow resistance were performed with oscilation method. After 4 week exposure, specific antibodies against OA are measured in serum and bronchoalveolar fluid by a direct enzyme immunoassay. The first bronchial reaction during exposure were observed on the 8-10th day. Intensity of reaction depended on the concentration of OA and was independent of N02 exposure. The relationships between OA-specific antibodies in serum and bronchoalveolar fluid and the intensity of bronchial reaction are under present investigation.