Investigation if Pathogenesis of SSPE Using Molecular Biological Techniques.

在之前的研究中，利用生物标记DNA探针的原位杂交方法成功地研究了麻疹病毒基因组在SSPE脑组织中的定位。然而，为了促进这项技术的进一步推广，必须提供更直接和客观的证据来证明这一技术的有效性。本研究以人T细胞白血病病毒I型(HTLV-I)为研究对象，建立了聚合酶链式反应-非放射性同位素检测系统。HTLV-I作为前病毒基因组DNA整合于核内，检测时具有病原性，与艾滋病或自身免疫性疾病有关。首先，在母婴传播中，每5例中有2例脐血单个核细胞扩增出HTLV-I特异性条带，提示该病毒经胎盘传播。第二，在HTLV-I中枢神经系统感染，即HTLV-I相关性脊髓病中，从微量脑脊液样本中扩增出非常强的信号，提示该病毒处于感染活跃状态，该系统已有效地应用于临床研究。随着更多的改进，非放射性同位素原位杂交将在临床研究中分享更重要的部分。

In a preceding study, a method of in situ hybridization using biotinlabelled DNA probe was successfully applied to investigate localization of measles virus genome in SSPE brain tissue. However, to facilitate further a spread of this technique, more direct and objective evidence of availability must be shown. For this purpose, polymerase chain reaction-non-radioisotopic detection system was constructed.Human T-cell leukemia virus type I (HTLV- I) was used as a target of this study, because, it was integrated in nuclei as proviral genome DNA, pathognomonic when detected, and shed on light in relation to AIDS or autoimmune diseases.First, in mother to child transmission, transplacental transmission of this virus was suggested by a fact that there existed 2 out of 5 cases in which HTLV-I specific bands were amplified from cord blood mononuclear cells.Second, in central nervous system infection of HTLV-I, that is, HTLV-I associated myelopathy, an active state of infection of this virus was suggested by a fact that remarkably strong signals were amplified from minute amount CSF samples of these patients.As mentiond above, this system was efficiently applied to clinical research. With more modifications, non-radioisotopic in situ hybridization will share a more important part of clinical investigations.