Pulmonary Hypoplasia and Morphological Characteristics and Surfactant Content Following Oligohydramnios or Diaphragmatic Hernia.

为了研究羊水过少对胎兔肺生长和肺生化发育的影响，在妊娠第23天将羊水通过导管插入母体腹膜腔排出。未进行羊膜分流术的1合生胎作为对照。胎儿于妊娠第28天腹产。在8例妊娠中，17例胎儿行羊膜分流术，22例胎儿作为对照。羊水分流术使羊水体积显著减少。两组胎儿体、肝、脑的湿重均无差异。然而，与对照组相比，羊水显著降低了胎肺湿重、胎肺湿重/体重比和每肺蛋白质浓度。在胎儿肝脏和脑组织中，每g湿组织和每mg蛋白质的总磷脂、磷脂酰胆碱（PC）或不饱和磷脂酰胆碱（dsc，活性表面活性剂的主要成分）浓度未见变化。羊膜分流胎肺中PC和dsc含量明显高于正常胎，且IIS比值高于正常胎。上述结果提示，羊膜沉默引起的羊水过少引起胎兔肺发育不全，但使胎兔肺表面活性物质含量升高。

To study the effect of oligohydramnios on lung growth and biochemical lung development in fetal rabbits, amniotic fluid was drained through a tube inserted into the maternal peritoneal cavity on the 23rd day of gestation. 1-ittermate fetuses without an amniotic shunt were used as controls. The fetuses were delivered abdominally on the 28th day of gestation. In a total of 8 pregnant does, 17 fetuses underwent amniotic shunting and 22 fetuses were used as controls. The amniotic shunt produced a significant reduction in the amniotic fluid volume. There were no differences in the wet weights of the fetal body, liver or brain between the two groups. however, the amniotic sliurit significantly decreased the wet weight of the fetal lung, fetal lung wet weight/body weight ratio, and protein concentration per lung as compared to tile control fetuses. In the fetal liver and brain tissues, no changes were found in the concentrations, of total phospholipids, phosphatidylcholine (PC) or disaturated phosphatidylcholine (DSPC, the main component of Iiing surfactant) per g of wet tissue and per mg of protein. however, the lungs of the fetuses with amniotic shunts contained significantly more PC and DSPC, and the IIS ratio was higher tlian in the control fetuses. These results suggest that the oligoliydramnios produced by an amniotic silunt causes pulmonary hypoplasia, but raises the pulmonary surfactant content of fetal rabbit lung.