Analysis of Oncogene Amplification and C-Myc Overexpression in Human Ovarian Cancer

对55例人卵巢癌组织中癌基因的扩增和表达进行了研究。用凝胶内再生法筛选性质未知的扩增基因，用Southern blot杂交法检测c-myc、Ki-ras和new癌基因的扩增情况。所有病例均未发现明显的基因扩增。通过Northern杂交检测，29例（52.7%）c-myc基因过表达，k-ras和neu基因均未过表达。浆液性囊腺癌和子宫内膜样癌组织中c-myc基因的过表达量是其他组织学类型肿瘤的3倍以上。免疫组化和原位杂交显示肿瘤细胞c-myc基因和蛋白均过表达。虽然其表达水平与恶性分级无关，但提示c-myc基因参与了卵巢肿瘤的早期癌变。

Amplification and expression of oncogenes were investigated in 55 cases of human ovarian cancer tissues. Gene amplificaion of unknown nature was screened by in-gel renaturation assay and amplification of the c-myc, Ki-ras and neu oncogenes were determined by Southern blot hybridization. No significant gene amplification was found in all the cases. By Northern hybridization assay, the c-myc gene was overexpressed in 29 cases (52.7%) whereas either k-ras or neu was not.The overexpression more than 3-fold of the c-myc gene was significant in serous cystadenocarcinoma and endometrioid carcinoma tissues compared with other histological types of tumor. Immunohistochemistry and in-situ hybridization revealed that tumor cells overexpressed both the c-myc gene and the protein.Although the expression level was not related to grading of malignancy, it is suggested that the c-myc gene is involved in early stages of carcinogesis of ovarian tumors.