Study on Autoregulatory Mechanism of Body Fluid with Special Emphasis on Kidney and Hormones

为了维持体液平衡，渗透压、电解质、酸碱平衡、营养物质、水分等自动调节机制在人体内起着重要作用。为了阐明自身调节机制，特别是肾功能和激素作用，本研究项目组成立。结果揭示了大鼠肾集合管加压素敏感性水通道的一级结构、噻嗪或阿米洛利与PTH在刺激家兔连接小管Ca ~（2+）吸收中的相互作用、激素受体及其信号转导在沿着大鼠肾单位的定位、大鼠肾小球系膜细胞中血管紧张素介导的信号转导、系膜功能和氯离子，加压素诱导的大鼠延髓集合管信号转导、NO诱导的大鼠降压和利钠/利尿、醛固酮受体的结构/功能关系、近端小管质子共转运体对药物转运的调节以及内皮素诱导的血管内皮细胞和平滑肌细胞信号转导。

To maintain body fluid homeostasis, autoregulatory mechanism including osmolarity, electrolytes, acid-base balance, nutritents, water etc, plays important roles in human body. To elucidate autoregulatory mechanism, especially kidney function and hormone actions, the present research project team was organized. The results revealed the primary structure of a novel vasopressin-sensitive water channel from rat renal collecting duct, interaction of thiazide or amiloride with PTH in stimulating Ca2+ absorption in rabbit connecting tubulus, localization of hormone receptors and their signal transductions along rat nephron, angiotensin-mediated signal transduction in rat glomerular mesangial cells, mesangial function and chloride ions, vasopressin-induced signal transduction of rat medullary collecting duct, NO-induced hypotension and natriuresis/diuresis in rat, structure/function relationship of aldosteron receptor, regulation of proton cotranspoter for drug transport in proximal tubulus, and endothelins-induced signal transduction in vascular endothelial and smooth muscle cells.