Investigation into the Development of Immunobinding Test Kit for Detecting Avian Mycoplasma Antibodies

建立了一种免疫结合（IB）检测试剂盒，用于检测鸡败支原体（MG）和滑膜支原体（MS）的血清抗体，以代替常规的凝集抗原，后者经常引起非特异性反应。将市售的10孔塑料板和两种硝化纤维素膜作为MG和MS抗原进行了比较。结果表明，塑料板处理起来更方便，但2周内出现的反应斑就消失了。而硝化棉条上出现的阳性斑点保存2年以上，无变色现象。IB反应比HI反应更敏感。前一种方法在接种两种支原体后1周能在所有SPF鸡中检测到MG或MS抗体，而后一种方法需要2周才能在所有接种过支原体的鸡中检测到抗体。试验结果表明，如果市面上有固定MG和MS抗原和试验试剂的试验试剂盒，则可在养鸡场常规进行IB试验。

An attempt was made to develop an immunobinding(IB)test kit for detecting serum antibodies against Mycoplasma gallisepticum(MG)and V. synoviae(MS)in lieu of routine agglutination antigen which frequently gives rise to unspecific reaction. Commercially available plastic plates with 10 wells and two sorts of nitrocellulose membranes were compared as the MG and MS antigens. It was revealed that the plastic plate were more convenient to handle, although developed reaction spots disappeared within 2 weeks. in contrast, positive spots appered on nitrocellulose strips had been kept for more than 2 years without discolarization. The IB reaction was more sensitive than the HI test. The former test could detect MG or MS antibodies in all of 10 SPF chickens one week postinoculation with either of the two mycoplasmas, whereas two weeks were required for for the latter test to detect antibodies in all inoculated birds. The test results have indicated that, if a test kit including immobilized MG and MS antigens and test reagents are commercially available, the IB test can be routinely carried out on chicken rtaising farms.