Studies on the Isolation and Biological Functions of Amphibian Hypothalamic Hormones

在两栖动物中，它仍然是未知的，因为它的性质仍然是一种刺激了从沥青地球上释放出的各种荷尔蒙。要识别那些高分子荷尔蒙，这是很重要的，要确定在纳米谱水平上的微量荷尔蒙的数量。我们已经分离了两种糖蛋白激素、卢托宾（LH）和氟托宾（FSH），并确定了两种激素的完整氨基酸序列。最近，我们也从牛蛙、拉纳·卡特斯贝亚那分离了第三种糖蛋白激素、甲状腺素（TSH）。我们已经建立了用于LH、FSH和TSH的常见Alpha亚单位的放射免疫测定系统，以及LH和FSH的特定β亚单位。我们也有来自牛蛙体液的分离性和/或神经中间性斑点的分离性，并确定了牛蛙体液的氨基酸序列，刺激了从肾上腺皮质激素的肾上腺素和皮质激素的释放。 ... More 和Xenopus Laevis。其中一种物质已被识别为49种氨基酸居留肽，从证明性过敏原的N-终端部分衍生出来，终止为NPP。其他肽被识别为Arg-vasotocin及其相关肽，被识别为Hydrin-1和Hydrin-2。我们也被孤立的肽活跃在从Hydrin 1和2中提取的神经中间层环的西诺普斯具有Hydrin 1和2中的中间结构的神经中间层，并被暂时用作Hydrin 1·1/2。However we could not isolate the adrenocorticotropin （ACTH） during the course of the isolation of those hormones。根据类似的方法，我们从鸡的沥青土地上分离了ACTH。根据类似的方法，我们从鸡的沥青土地上分离了ACTH。因此，在肺结核中存在可能性，而NPP是ACTH的功能替代品。无线电免疫测定系统的建立正在进行中，从大约1， 000只牛蛙中提取出1 M Acetic acid的高硫醚，并在Sephadex-G25柱上进行色谱分析，然后在DEAE离子交换柱上进行分析。Gonadotropin释放荷尔蒙（GnRH，由α亚单位的放射免疫测定测量）已被提炼成0.3- 0.5 M NaCl。GnRH的最终隔离和识别正在进行中。Less（低）

In the amphibia, it is still remain unknown for the nature of the hypothalamic hormones which stimulate the release of the various hormones from the pituitary gland. To identify those hypothalamic hormones, it is important to determine the amount of the pituitary hormones in qualitatively and quantitatively in nanogram level. We have already isolated the two glycoprotein hormones, lutropin (LH) and follitropin (FSH), and determined the complete amino acid sequences of both hormones. Very recently, we also isolated the third glycoprotein hormone, thyrotropin (TSH) from the bullfrog, Rana catesbeiana. We have established the radioimmunoassay systems for the common alpha-subunit for LH, FSH and TSH, and specific beta-subunits of LH and FSH. We have also isolated from the distal lobe and/or neurointermediate lobe of bullfrog pituitary, and determined the amino acid sequences of the substances which stimulate the release of aldosterone and corticosterone from the adrenal cortex of bullfrog … More and Xenopus laevis. One of those substances is identified as 49 amino acid residue peptide, derived from the N-terminal part of the proopiomeranocortin, termed as NPP. The other peptides are identified as Arg-vasotocin and its related peptides, termed as Hydrin-1 and Hydrin-2. We also isolated a peptide which is active in release of aldosterone from the neurointermediate lobe of pituitary gland of Xenopus having the intermediate structure of Hydrin 1 and 2, and tentatively termed as Hydrin 1・1/2. However we could not isolate the adrenocorticotropin (ACTH) during the course of the isolation of those hormones. According to the similar methods, we have isolated the ACTH from the pituitary gland of chicken. According to the similar methods, we have isolated the ACTH from the pituitary gland of chicken. Thus there is a possibility that in amphibia, NPP is the functional substitute of ACTH. The establishment of the radioimmunoassay systems are under progress.The extract from about 1, 000 hypothalamus of the bullfrog with 1 M acetic acid is gel filtrated on Sephadex-G25 column, then chromatographed on DEAE ion-exchange column. The gonadotropin-releasing hormone (GnRH, measured by radioimmunoassay for the alpha-subunit) was eluted with 0.3-0.5M NaCl. The final isolation and identification of the GnRH is under progress. Less