Acid mucopolysaccharide metabolism and bone formation in fish

1)从正常虹鳟鱼的筛骨和椎体中提取酸性粘多糖，采用hplc -纤维素柱色谱法、醋酸纤维素电泳法和醋酸纤维素电泳法测定。chondroitinase消化。软骨素和透明质酸是筛骨酸性粘多糖的主要成分。硫酸软骨素是脊椎骨中主要的酸性粘多糖。2)以平均体重0.3g的虹鳟鱼饲喂抗坏血酸（AsA）缺乏和补充饲料15周。asa缺乏组的鱼在饲养第12周开始出现典型的坏血病症状，如前凸和脊柱侧凸。asa缺乏组的全身酸性粘多糖含量显著高于asa补充组。asa补充组的非硫酸粘多糖含量随着年龄的增长而下降，而asa缺乏组的含量则没有下降。两组在出现明显坏血病症状前椎体酸粘多糖含量基本相同，而asa缺乏组在坏血病晚期椎体酸粘多糖含量急剧增加。两组间酸性粘多糖中硫酸氢黄素4和硫酸氢黄素6的含量比无显著差异。然而，asa缺乏组的软骨素和透明质酸含量高于asa补充组。

1)Acid mucopolysaccharides were extracted from the ethmoid bone and vertebra of normal rainbow trout, and determined by CPC-cellulose column chromatography, electrophoresis on cellulose acetate and. chondroitinase digestion. Chondroitin and hyaluronic acid were the major components of acid mucopolysaccharides in the ethmoid bone. Chondroitin sulfates were the major acid mucopolysaccharides in the vertebra.2)Rainbow trout weighing 0.3g on average were fed ascorbic acid(AsA)-deficient and AsAsupplemented diet for 15 weeks. The fish of AsA-deficient group began to show the typical signs of scurvy, such as lordosis and scoliosis, at the 12th week of rearing. The whole body acid mucopolysaccharides contents were considerably higher in the AsA-deficient group than in the AsA-supplemented-group. The contents of non-sulfated acid mucopolysaccharides decreased in the AsA-supplemented group with age but not decreased in the AsA-deficient group. The vertebral acid mucopolysaccharides contents in both groups were the same level before they developed the apparent signs of scurvy, whereas the contents in the AsA-deficient group increased extremely in the advanced stages of scurvy. There were no difference between both groups in the content ratios of chodroitin 4-sulfate and 6-sulfate of the acid mucopolysaccharides. However, the contents of chondroitin and hyaluronic acid of AsA-deficient group were higher than those of AsA-supplemented group.