Regulation Mechanism on Growth and Differentiation of Pancreatic Cancer Cells

本研究以作者建立的仓鼠和人胰腺癌细胞系为实验模型，<TM>通过形态学观察和BrdU累积标记法细胞动力学分析，研究了基底膜基质（MATRIGEL^）对仓鼠和人胰腺癌细胞系的诱导分化作用。基底膜基质诱导的胰腺癌细胞分化后，电镜下可见细胞间隙连接增多，细胞内Ca ~（++）和cAMP定量结果不确定，细胞内Ca ~（++）和cAMP定量结果不确定。当注射到同基因动物中时，与对照细胞相比没有显着差异。这一结果表明，基底膜基质诱导胰腺癌细胞分化是可逆的，为胰腺癌细胞生物学的进一步研究提供了实验依据。

The results of the study using experimental pancreatic cancer models, hamster and human cell lines established by the authors, were summarized as follows;1) Basement membrane matrix (MATRIGEL^<TM>) induced the differentiation of pancreatic cancer cell lines, both of hamster and human origin, by morphological study and cell-kinetic analysis using BrdU cumulative labeling method.2) The influences of various gut peptides, steroid hormones, and growth factors on growth and morphology of pancreatic cancer cells depended on each cell line.3) The differentiated cancer cells induced by basement membrane matrix showed increased number of gap junctions by electron-microscopy.4) Results of quantification of intracellular Ca++ and cyclic AMP were indefinite.5) In vivo growth characteristics of the differentiated hamster pancreatic cancer cells by basement membrane matrix, showed no significant difference compared with control cells, when injected into syngeneic animals. This result indicates that differentiation-induction by basement membrane matrix is reversible.These results should contribute to the further progress in the research of pancreatic cancer cell biology.