The study of disturbance on pulmonary endothelial cell during lung allograft rejection

为了研究同种异体肺移植急性排斥反应中内皮细胞损伤与特异性免疫反应的关系，我们研究了供体受体PBL特异性细胞毒活性和移植肺的组织学变化。结果发现，在细胞毒性活性增加的同时，肺泡腔中有渗出物，这似乎是由内皮细胞的高渗透性引起的。内皮细胞生存能力的评估是研究排斥反应所致肺损伤的必要条件。研究了缓激肽诱导常温保存损伤的狗肺内皮细胞释放前列腺素I2的变化及其与体内移植肺气体交换功能的相关性。发现损伤肺PGI2释放能力明显降低。这被认为是有用的测定内皮细胞的活力。

Histological changes of the grafted lungs during acute lung allograft rejection To investigate the relation between the injury to endothelial cells and specific immunoresponse during lung allograft rejection, we studied for donor specific cytotoxic activity in recipient PBL and histological changes of the grafted lung. It was found that there was exudation, which appeared to be caused by hyperpermiability of endothelial cells, in the alveolar space at the time of increased cytotoxic activity. It is considered that an evaluation of viability in endothelial cells is essential to study for lung injury due to rejection.Changes of Prostaglandin I2 release capacity from dog lungs PGI2 releasing capacity induced by bradykinin from endothelial cells of dog lung which were injured by normothermic preservation, and correlation between the changes in vitro and gas exchanie function of grafted lung in vivo was investigated. It was found that PGI2 releasing capacity in injured lungs showed significant decrease. It is considered that this assay is useful to estimate the viability of endothelial cells.