Experimental Study of Lung Preservation -from the viewpoint of the changes of anionic sites

本实验观察了大鼠肺基底膜保存过程中阴离子部位的变化，分为4组：A组：对照组；B组：常温肝素化生理盐水保存；C组：37ﾟC肝素化生理盐水保存；D组：37ﾟC保存EP3液。阴离子位染色后，我们观察切片上的情况。结果：10.2Lt；+-lt；1B组：12.6；+-lt；1C组：12.0；+-lt；+-gt；1D组：11.8&lt；+-lt；+-gt；结论与A组(对照组)相比，B、C、D组阴离子SIT密度显著增加(P&lt；0.05)，需要更多的实验来对这些结果进行准确的生理解释。

We studied the changes of anionic sites produced by preservations in basement membrane of lungs using Wister rats.They were divided into 4 groups as follows ;A group : controlB group : stored at room temperature in heparinized salineC group : stored at 37ﾟC in heparinized salineD group : stored at 37ﾟC in EP3 solutionSmall tissue blocks were taken from the lungs and anionic sites were stained using strong cationic polyethyleneimine as a tracer following Schurer's method. After staining of anionic sites, we observed the sections on. electrone microscopy and caliculated the dot density of basement membrenes(dot number / cm at the magnification of 15410)and analyzed them by-the system(Hard : NEC 9801 VX, Soft : Nikon Cosmozone).RESULTSA group : 10.2<plus-minus>1B group : 12.6<plus-minus>1C group : 12.0<plus-minus>1D group : 11.8<plus-minus>1.8 (mean<plus-minus>S.CONCLUSIONCompared to the A group(control), B, C and D group hdd higher density of anionic sit significantly(p<0.05)We need more experiments to put an exact physiological interpretation on these results.