Analysis of structure of mucin type glycoprotein seaeted from endometrial Carcinoma

子宫内膜癌细胞产生一种高分子量粘蛋白糖蛋白(MGP)。在子宫内膜癌患者血清中检测到该MGP。我们研究了子宫内膜癌培养细胞分泌的MGP的结构，半纯化表明MGP是一种高分子物质，不含末端唾液酸。用与已定义的碳水化合物结构发生特异性反应的各种单抗分析其糖类结构，经高效液相色谱和免疫亲和层析等高纯化步骤后，用Edman‘s氨基序列分析多肽部分。结果表明，MGP由高度岩藻糖化的聚乳糖胺碳水化合物结构和约相对分子质量的核心肽组成。40k Dalton。在多肽EMBO数据库中没有注册同源的多肽结构，但推测的氨基酸序列与胰腺弹性蛋白酶III有很高的同源性。这表明MGP与胰腺弹性蛋白酶III部分共享一个古老的基因。

Endometrial carcinoma cells produce a high molecular weight mucin type glycoprotein (MGP). This MGP was detected in sera of patients suffering from endometrial carcinoma. We investigated the structure of MGP secreted from endometrial carcinoma culture cell line.Semi-purification showed that MGP was high molecular material and there were no terminal sialic acids. The carbohydrate structure was analysed by various monoclonal antibodies which specifically react to previous defined carbohydrate structures, and peptide portion was investigated by Edman's amino sequence analysis after high purification procedures by high performance liquid chromatography and immunoaffinity column. It was elucidated that MGP was composed of highly fucosylated poly lactosamine carbohydrate structure and core peptide of approximately MW. 40K dalton.There were no homology peptide structures registered in peptides EMBO data base but the putative DNA sequence of the amino sequence had high homology with pancreatic elastase III.This results suggest that MGP partially share an ancient gene with pancreatic elastase III.