Suppression of corneal graft rejection in rabbits by local administration of immunosuppressive agents.

角膜移植排斥反应是角膜移植手术失败的主要原因之一。本研究旨在探讨局部应用环孢素(CyA)和FK-506对兔角膜移植排斥反应的抑制作用。在对兔实验性角膜移植进行研究之前，我们测量了两种药物单次给药50次后在角膜中的浓度。角膜中CyA浓度在滴眼后30分钟达130 ng/gR，24小时达70 ng/gR。另一方面，FK-506在角膜中的浓度在注射后30分钟达到370 ng/gR，在24小时达到130 ng/gr。I组(0.025%环磷酰胺滴眼液)10眼，II组(0.05%环磷酰胺滴眼液)，III组(0.1%FK-506滴眼液)，IV组(FK-506 0.1 mg/kg结膜下注射，每周1次)，V组为对照组。术后50d内每日给药4次，移植排斥反应：I组4只眼，II组2只眼，III组10只眼无一例，IV组1只眼，对照组8只眼。0.05%环磷酰胺滴眼液、0.1%FK-506滴眼液和FK-506结膜下注射均能明显抑制角膜移植排斥反应。

Corneal graft rejection is one of the leading causes of failure in corneal graft surgery. The purpose of our research is to find the efficacy on the suppression of corneal graft rejection in rabbits by local administration of cyclosporin (CYA) and FK - 506. Before we study the experimental corneal grafting of the rabbits, we measured the concentration of the two drugs in the cornea after 50 mul single application. The concentration of CYA in the cornea reached 13OOng/gr at 30 minutes and 7OOng/gr at 24 hours after the instillation. On the other hand, the concentration of the FK - 506 in the cornea reached 370ng/gr at 30 minutes and 130ng/gr at 24 hours after the instillation.Fifty animals received corneal allografts and skin grafts were also exchanged between pairs 14 days after the keratoplasty. Group I (10 eyes) was 0.025% CYA eye drops, Group II (10 eyes) was 0.05 % CYA eye drops, Group III (10 eyes) was 0.1 % FK - 506, eye drops, Group IV (10 eyes) was FK-506 (0.1mg/kg) subconjunctival injection (once a week), Group V was control (no medication). All eyes were give 4times a day during 50 days after the surgery, graft rejection was found 4 of 10 eyes in Group I, 2 of 10 eyes in Group II, none of 10 eyes in Group III, one'of 10 eyes in Group IV and 8 of 10 eyes in control. 0.05% CYA eye drops, 0.1 % FK - 506 eye drops and FK - 506 subconjunctival injection were significantly suppressed the corneal graft rejection.