Three Dimensional Reconstruction of the Brain in the House Musk Shrew

本研究的目的是确认最近被驯化为实验性若虫的家麝鼩脑中的核团和束，并显示这些核团和束的三维排列。用HRP逆行标记法对细胞核或亚核进行了鉴定。在个人计算机上使用三维重建软件OZ（Olympus，Japan）显示细胞核的立体图像。家麝面神经核体视学显示，面神经核可分为内侧、外侧、背侧、中间和副五个亚核。HRP法研究表明，外周肌的背腹和口尾排列分别反映在核内的背腹和外内侧亚核上. X、XII神经传入根走腹外侧，不与从背外侧进入三叉神经脊束核的VII、X神经传入根汇合。迷走神经迷Ⅲ、Ⅳ、Ⅵ核在类似啮齿类动物的部位呈断续的细胞柱。

The Purpose of this study was to confirm the nuclei and tracts in the brain of the house musk shrew, which was recently domesticated as an experimental nimal, and to show three dimensional arrangement of those nuclei and tracts. Identification of the nuclei or subnuclei was studied by the retrograde marking method using the HRP. Stereoscopic picture of the nuclei was shown using the three dimensional reconstruction soft ware OZ (Olympus, Japan) on a personal computer.1. Stereoscopic demonstration of the facial nucleus of the house musk shrew showe clearly five subnuclei (medial, lateral, dorsal, intermediate and accessory). The HRP studies revealed that dorsal-ventral and oral-caudal arrangements of peripheral muscles reflected on the subnuclei supplying these muscles as dorsal-ventral and lateral-medial in the nucleus, respectively.2. Afferent radices of X and XII ran ventrolaterad without uniting with afferent roots of VII and X nuclei which entered from dorsolateral side and ran through the spinal trigeminal nucleus. Periambigual area was there in the ventro-medial region of the ambiguous and afferent vagal radix.3. The III, IV and VI nuclei showed intermittent cell columns at the place resembling to the rodents.