Physiological role of cytokines on homeostasis

1)IL-1产生：采用原位杂交和免疫组织化学技术，研究了在细菌内毒素（脂多糖，LPS）作用下，家兔终板血管器（OVLT）是否产生白细胞介素-1（IL-1）。为了合成用于原位杂交研究的反义和正义RNA探针，对兔IL-1 β DNA进行亚克隆。用<35>RNA聚合酶转录1 S标记的RNA探针。静脉注射LPS（4 μ g/kg）引起发热时OVLT产生IL-1 β RNA。IL-1与CRF：本研究探讨了中枢皮质激素释放因子（CRF）在心血管和热性脑病发生发展中的作用。 ...更多信息 l应激或IL-1 β自由活动大鼠诱导的反应。侧脑室（icv）注射（10 μ g）α-螺旋CRF（CRF受体拮抗剂），可显著减轻由转换笼应激引起的高血压、心动过速和体温升高。而侧脑室注射α-螺旋CRF对（PG）E_2引起的高血压、心动过速、发热无明显影响。相反，icv注射α-螺旋CRF可减轻icv注射IL-1 β引起的高血压、心动过速或发热。这些结果表明，中枢CRF在笼舍应激反应的发生中起重要作用，但它似乎与ip IL-1 β或中枢PGE_2引起的高血压、心动过速和发热无关。3）IL-1和ACTH：进行该研究以研究腹膜内（IP）注射吲哚美辛预处理的效果，吲哚美辛是前列腺素合成的抑制剂，心理应激诱导的反应，包括心血管，体温调节和激素反应在自由移动的大鼠。心理应激由转笼应激引起。当老鼠被替换到新的环境中后，血压、心率和体温升高。应激30分钟后，血浆促肾上腺皮质激素（ACTH）和前列腺素E^2水平高于正常水平。吲哚美辛还能抑制由笼转换应激引起的血浆ACTH和PGE_2水平的升高。这些结果表明，三尖杉酯碱参与了由心理应激引起的体温过高和ACTH反应的发展。少

1) IL-1 production : the study was carried out to determine whether interleukin-1 (IL-1) production occurs in the rabbit organum vasculosum laminae terminalis (OVLT) during bacterial endotoxin (lipopolisaccharide, LPS), using in situ hibridization and immunohistochemical techniques. For the synthesis of antisense and sense RNA probes for insitu hybridization study, the rabbit IL-1betacDNA was subcloned. ^<35>S-labeled RNA probes were transcribed with RNA polymerase. The intravenous injection of LPS (4 mug/kg) caused the production of IL-1betamRNA in the OVLT during fever. Some cells ajacent to blood vessels in the brain and in the pia mater also showed IL-1betamRNA expression, although the expression was somewht lower than that seen in the OVLT.These results support the hypothesis that IL-1 is produced in the brain during fever.2) IL-1 and CRF : the study was done to examine about the role of central corticontropin-releasing factor (CRF) in the development of caridiovascular and therma … More l responses induced by stress or by IL-1beta free-moving rats. Intracerebroventricular (icv) injection (10mug) of alpha-helical CRF (CRF receptor antagonist), significantly attenuated hypertension, tachycardia, and a rise in body temperature induced by cage-switch stress. However, icv injection of alpha-helical CRF had no effect on hypertension, tachycardia, of fever induced by prostagland in (PG) E_2. In contrast, icv injection of alpha-helical CRF attenuated hypertension, tachycardia, or fever induced by icv injection of IL-1beta. These results suggest that central CRF has an important role in the development of the cage-switsh stress-induced responses, but it does not seem to contribute to the hypertension, tachycardia, and fever induced by ip IL-1beta or by central PGE_2.3) IL-1 and ACTH : the study was done to investigate the effect of pre-treatment with intraperitoneal (ip) injection of indomethacin, an inhibitor of prostaglandin systhesis, on psycological stress-induced responses including cardiovascular, thermoregulatory and hormonal responses in free-moving rats. Psychological stress was induced by cage-switch stress. After the rats wereplaced in the novel environment, blood pressure, heart rate and body temperature increased. Plasma levels of adrenocorticotropic hormone (ACTH) and PGE^2 were higher 30min after exposure to stress, in comparison the nornal levels. Indomethacin also suppressed the increases in the plasma levels of ACTH and PGE_2 induced by cage-switch stress. These results suggest that prostaglandins are involved in the development of hyperthermia and the ACTH response induced by psychological stress. Less