FORENSIC, BIOCHEMICAL AND GENETIC STUDY ON GENOTYPES OF BLOOD GROUPS

(1) DNA表型测定方法的建立建立了一种简单可靠的从DNA样品中测定o基因的新方法。采用sds -蛋白酶k -酚法或chelex-100法提取DNA。采用等位基因特异性聚合酶链反应扩增o基因。用15% page和硝酸银染色检测扩增的DNA。无亲缘关系的25例0型人DNA中有0基因产物，10例ab型人DNA中无0基因产物。(2)血清学研究abo表型及家族分析3年共采集19个家族65份样本，无亲缘关系385份样本。我们的血清学和家庭研究中没有aa或bb基因型的人。(3) abo系统中基因序列中影响抗原表达的因子。在这一课题上，目前尚无研究结果。我们需要更多的研究来得到答案。

(1) DEVELOPMENT OF PHENOTYPE DETERMINATION METHOD FROM DNAWE DEVELOPED A NEW SIMPLE AND RELIABLE METHOD FOR THE DETERMINATION OF O-GENE FROM DNA SAMPLES.DNA WAS EXTRACTED BY SDS-PROTEINASE K-PHENOL METHOD OR BY CHELEX-100 METHOD.O-GENE WAS AMPLIFIED BY ALLELE SPECIFIC POLYMERASE CHAIN REACTION.THE AMPLIFIED DNA WAS DETECTED BY 15% PAGE AND SILVER NITRATE STAINING.DNA FROM UNRELATED TWENTY-FIVE O PHENOTYPE PERSONS HAD O-GENE PRODUCT, AND THOSE FROM TEN AB PHENOTYPE PERSONS HAD NO PRODUCT.(2) SEROLOGIC STUDY OF ABO PHENOTYPE AND FAMILY ANALYSISSIXTY-FIVE SAMPLES FROM NINETEEN FAMILIES AND UNRELATED THREE HUNDRED EIGHTY-FIVE SAMPLES WERE COLLECTED IN THESE THREE YEARS.THERE WAS NO AA OR BB GENOTYPE PERSON FROM OUR SEROLOGIC AND FAMILY STUDIES.(3) FACTOR(S) AFFECTING ANTIGEN EXPRESSION FROM GENOME SEQUENCE IN THE ABO SYSTEMWE HAVE NO RESULT ON THIS SUBJECT IN THIS PERIOD.WE NEED MORE STUDIES TO GET THE ANSWER.