Studies on Proliferative Hybrid Artificial Liver Using Lipophilic Membranes.

亲脂性毒素是引起肝昏迷的强效药物，本研究采用脂膜技术对其进行选择性排除。采用石蜡油处理后的聚丙烯和聚乙烯中空纤维膜。苯酚和二甲基硫化物（DMS）是众所周知的亲脂毒素，可以很好地通过这些亲脂膜，但这些代谢物是亲水的，不能通过这些膜。采用肝微粒体进行DMS氧化反应，并应用于人工肝脏系统。包封的肝细胞也被应用于苯酚的葡萄糖醛酸化反应。肝微粒体和包被肝细胞都可以通过亲脂膜排除亲脂毒素。通过处理产生亲脂性毒素的动物，可使酶解毒能力提高30 ~ 50%。但没有添加剂对包被肝细胞的增殖和功能有促进作用。只有持续添加含有肝微粒体的溶液，亲脂膜人工肝支持的解毒单位才会增加。

Lipophilic toxins were known as highly potent agents which induce hepatic coma and selective exclusion of these toxins using lipid membrane technique was investigated in this study. Both Polypropylene and Polyethylene hollow-fiber membranes were used after paraffin oil treatment. Phenol and Dimethylsulfide(DMS) were well known lipophilic toxins and permeate through these lipophilic membranes well but these metabolites were hydrophilic and were not able to permeate through the membranes. Liver microsome was used for DMS oxidation reaction and applied to the system for artificial liver. Encapsulated hepatocytes were also applied to the system for glucuronidation reaction of phenol. Both liver microsome and encapsulated hepatocytes well utilized on exclusion of lipophilic toxins through lipophilic membranes. 30 to 50% of those ability on enzymatic detoxification for lipophilic toxins were accererated by treating the animals which are the source of them. But no additives were beneficial on proliferation and functions of encapsulated hepatocyte. Only continuous addition of the solution containing liver microsomes achieved increasing detoxification unit on artificial liver support using lipophilic membrane.