Development of hybrid artificial liver supprt system using lipophilic

亲脂性混合人工肝支持系统的开发

• 批准号: 05670983
• 负责人: YAMAMOTO Tetsu
• 金额: $ 1.41万
• 依托单位: ASAHIKAWA MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1993
• 资助国家: 日本
• 起止时间: 1993 至 1994
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-05670983/
• 关键词: Hyperplatic Nodule; Drug Metabolism; Artificial Liver Support; Lipophilic Membranes; Glucuronidation Reaction; Encapsulated Hepatocytes; 2-Acetylaminofluorene; 親指化膜; 門脈下大静脈吻合; Diethylnitrosamine

The utilities of encapsulated hepatocytes from hyperplastic nodule (HN) were studied on their detoxifying abilities as metabolic reactor on artificial liver support system. The cells of chemically induceced hypeplastic nodules exhibited 1.7 fold higher NADPH-Cyt.C Reductase activities than normal hepatocytes. And the activities were more induced on the animal with portacaval shunt through a mechanisum of enzyme induction. Combination of these two methods supposed to be beneficial on getting high performance cells for drug meabolisum. But the step of isolate liver cells from HN was not successful, and minced HN tissue were used for further studies. Encapsulated HN cells exhibited transmembranous phenol glucuronidation activities through lipophilic membranes. But the activity was not higher than expected. On morphological study, 70-80% of cultured encapsulated HN cells exhibited necrotic forms on 48 hours. And low oxigen perfusion in encapsulated HN cells was supposed to be the cause of their low activity. Development of efficient method for the isolation of HN cells was thought to be essential for utilizing the cells on artificial liver support.

研究了包埋增生结节（HN）肝细胞作为代谢反应器在人工肝支持系统中的应用。化学诱导增生结节的细胞表现出比正常肝细胞高1.7倍的NADPH-Cyt.C还原酶活性。门腔静脉分流术动物的上述活性通过酶的诱导机制被诱导。这两种方法的结合有利于获得高性能的药物代谢细胞。但从HN中分离肝细胞的步骤不成功，切碎的HN组织用于进一步的研究。包封的HN细胞通过亲脂性膜表现出跨膜苯酚葡萄糖醛酸化活性。但活动并没有高于预期。在形态学研究中，70-80%的培养包封的HN细胞在48小时表现出坏死形式。微囊化HN细胞的低氧灌注可能是其活性低下的原因。HN细胞分离方法的建立是HN细胞在人工肝中应用的关键。

项目成果

水戸廸郎: "21世紀の外科領域における研究" 外科. 56. 1011-1016 (1994)

水户周郎：“21世纪外科领域的研究” Surg. 56. 1011-1016 (1994)

T.Yamamoto and M.Mito: "Progress and Advance in Surgical Therapy-Progress in Liver Transplantation and Now-" Hokkaido J.Surg.38. 20-25 (1993)

T.Yamamoto 和 M.Mito：“外科治疗的进展和进展 - 肝移植的进展和现在 -”Hokkaido J.Surg.38。

山本 哲: "外科治療の進歩・変遷 「移植」-肝移植の進歩と現況を中心に-" 北海道外科雑誌. 38. 20-25 (1993)

山本聪：“‘移植’外科治疗的进展和变化 - 关注肝移植的进展和现状 -”北海道外科杂志 38. 20-25 (1993)。

葛西眞一: "先進医療:癌患者への臓器移植-肝癌を中心に-" 総合臨床. 43. 35-37 (1994)

Shinichi Kasai：“先进的医疗护理：癌症患者的器官移植 - 关注肝癌”一般临床实践。 43. 35-37 (1994)

山本哲: "Hyperplastic Nodule(HN)肝細胞の被包化によるモジュール作製の検討" 人工臓器. (発表予定).

Satoshi Yamamoto：“通过封装增生结节（HN）肝细胞来检查模块生产”人造器官（预定演示）。