Basic studies on the utility of progenitor cells in cord blood for artificial liver support.

脐带血中的祖细胞用于人工肝支持的基础研究。

• 批准号: 07807108
• 负责人: YAMAMOTO Tetsu
• 金额: $ 1.41万
• 依托单位: ASAHIKAWA MEDICAL COLLEGE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07807108/
• 关键词: Cord Blood; Hepatocyte Growth Factor (HGF); Artificial Liver Support; Stem Cell

The mononuclear cells in cord blood were studied as a source of liver support unit.Initially most effective method of high yield on CD34 positive cells was discussed. The choice of anti-coaglant agent was the most important factor on the recovery of CD34 positive cells. Heparin was not effective because its short anti-coaglant activities. Thus the ACD solution was recomended for harvesting coad blood.The serum level of HGF were 0.494<plus-minus>0.117ng/ml in cord blood (n=4) and 0.358<plus-minus>0.047ng/ml in peripheral blood (n=4). The released HGF levels in the culture medium (n=5) with cytocaines were 0.126<plus-minus>0.094ng/10^6 cells on 24 hours and 0.448<plus-minus>0.424ng/10^6 cells on 72 hours. And without cytocaines (n=5), the levels were 0.125<plus-minus>0.080ng/10^6 cells and 0.152<plus-minus>0.121ng/10^6 cells, respectively. The calculated HGF release values of CD34+CD33-cells with cytocaines were 13.3<plus-minus>11.3ng/10^6 cells on 24 hours and 41.2<plus-minus>31.7ng/10^6 cells on 72 hours.

本文对脐血单个核细胞作为肝支持单位的来源进行了研究，初步探讨了高产量CD 34阳性细胞的最有效方法。抗凝剂的选择是影响CD 34阳性细胞回收率的重要因素。肝素抗凝时间短，疗效不佳。结果表明，脐血和外周血中HGF含量分别为0.494 <plus-minus>± 0.117ng/ml和0.358 <plus-minus>± 0.047ng/ml。细胞毒作用24小时和72小时，细胞培养液（n=5）中释放的HGF水平分别为0.126 <plus-minus>± 0.094ng/10^6个细胞和0.448 <plus-minus>± 0.424ng/10^6个细胞。而在无细胞卡因的情况下（n=5），其水平分别为0.125 <plus-minus>± 0.080ng/10^6个细胞和0.152 <plus-minus>± 0.121ng/10^6个细胞。细胞毒作用24小时后，CD 34 + CD 33-细胞的HGF释放量为13.3 <plus-minus>± 11.3ng/10^6个细胞，<plus-minus>72小时后为41.2 ± 31.7ng/10^6个细胞。

项目成果

B.Jiang: "Enhancement of proliferation of intrasplenically transplanted hepatocytes in cimiotic rats by hepatic stiwbtay substance" Transplantation. 63(1). 131-135 (1997)

B.Jiang：“肝基质物质增强文麻大鼠脾内移植肝细胞的增殖”。

T.Yamamoto et al.: "HGF release from progenitor cells in cord blood."

T.Yamamoto 等人：“脐带血中的祖细胞释放 HGF。”

B.Jiang et al.: "Enhancement of proliferation intrasplenically transplanted hepatocytes in cirrhotic rats by hepatic stimulatory substance." Transplantation. 63 (1). 131-135 (1997)

B.Jiang 等人：“肝刺激物质增强肝硬化大鼠脾内移植肝细胞的增殖。”

B. Jiang: "Enhancement of proliferation intrasplenirally transplanted hepatocytes in cirrhotic rats by hepatic stinulatory substance" Transplantation. 63(1). 131-135 (1997)

B. Jiang：“通过肝刺激物质增强肝硬化大鼠脾内移植的肝细胞的增殖” 移植。

T. Yamamoto: "HGF release from progenitor cells in cord blood" Transfusion.

T. Yamamoto：“脐带血中祖细胞释放 HGF” 输血。