Morphogenesis of fibrous long-spacing collagen and tumor metastasis

应用免疫组织化学方法，对胶原酶处理的新生小鼠皮肤、神经母细胞瘤和培养成纤维细胞的纤维长间隔胶原(FLS)中的I、III、IV、V、VI型胶原、层粘连蛋白和纤维粘连蛋白的分布进行了电子显微镜分析，以阐明纤维的形态发生和起源。VI型胶原分布于直径为6 nm的细丝、基底板附近的未成形物质和胶原纤维周围以及纵向排列的纤丝中。在层粘连蛋白染色中，毛细血管基板和基底层细胞上的抗体反应较强。FLS的暗带为电子致密的非晶态物质，周期为100 nm，染色浓密，在神经鞘瘤中，我们观察到层粘连蛋白的存在，但没有观察到其他物质。层粘连蛋白分布于毛细血管基底层、许旺细胞的部分基底层和纤维母细胞的暗带。这一结果与布伦的研究相一致。对层粘连蛋白的分布研究表明，FLS的暗带可能是由胶原酶部分消化的基底板分解产物在含有VI型胶原的细丝上沉淀而成，其周期间隔约为90 nm。

We are analyzed the distribution of type I , III,IV,V,VI collagens, laminin and fibronectin in fibrous long-spacing collagen (FLS) of collagenase-treated newborn mouse skin, schwnnoma and cultured fibroblasts by immunohistochemistry at the electron microscopic level to clarify the morphogenesis and origin of the fibers.In the enzyme-treated mouse dermis, we observed the presence of type VI collagen and laminin. The distribution of type VI collagen were recognized in thin filaments with a diameter of 6 nm, unformed material in the vicinity of basal laminae and collagen fibers and the longitudinally aligned filaments of FLS.In staining of laminin, we observed the more positive reaction with the antibody on the basal laminae of capillaries and basal layr cells. The dark bands of FLS, which were electron-dense amorphous materials with a periodicity of 100nm, were stained densely and specifically.In the schwannoma, we observed the presence of laminin, but not the others. The distribution of laminin were recognized in basal laminae of capillaries, some basal laminae of schwan cell and dark bands of FLS.In the matrix of culltured fibroblasts, we observed the presence of type VI collagens. This results corresponded with the Brun's study. Studying of the distribution of laminin, there aren't laminin.These results indicate that the dark bands of FLS may be formed by precipitation of breakdown products from the demal basal laminae partially digested by collagenase at the type VI collagen-contained filaments with the periodic intervals of about 90nm.