The role of apoE from human monocyte in atherosclerosis

单核/巨噬细胞在动脉粥样硬化的发生发展中起重要作用。单核细胞生长形成细胞，使血管内膜增厚，即动脉粥样硬化。单核/巨噬细胞。载脂蛋白E的分泌被认为是动脉粥样硬化形成的负面因素，本研究旨在探讨载脂蛋白E的分泌剂量与动脉粥样硬化形成的关系。通过离心和塑料贴壁技术从外周血中分离纯化的人单核细胞，在95%的氧气和5%的二氧化碳条件下培养5d，用特异性抗载脂蛋白E抗体用免疫学方法测定培养上清液中的载脂蛋白E。采用酶法测定培养的单核/巨噬细胞中胆固醇和胆固醇酯的含量。在健康志愿者中，单核/巨噬细胞分泌载脂蛋白E和摄取胆固醇存在较大的个体差异。这些结果推测，分泌载脂蛋白E和储存的胆固醇可能是未来怀疑动脉粥样硬化发展的一个特异和敏感的标志物。分泌型载脂蛋白E测定的意义还有待长期的随访研究。我们开始研究临床状态下分泌型载脂蛋白E的测定意义。首先，我们对糖尿病(DM)进行评估，因为DM是一种常见病，我们可以从患者的周围血管中获得大量的血液。由于真菌和细菌的污染，我们在培养单核/巨噬细胞时遇到了很多麻烦。我们无法理解是什么因素影响了这些麻烦。没有实验室标记物，如血糖、HbAlc、C肽和/或尿糖与疾病、污染没有直接关系。我们继续在糖尿病实验室进行这项研究，如果可以的话，我们希望评估apoE在急性心肌梗死和脑梗塞这两种动脉粥样硬化的典型临床中的意义。

Monocyte/macrophage plays an important role in developing atherosclerosis. Monocyte would growing form cell, increasing the intima of the vessel, that was atherosclerosis. MOnocyte/macrophage. Secreting apoE was considered the negative factor of developing atherosclerosis This project has a purpose of evaluation the relationship between the dose of secreting apoE and developing atherosclerosis.Human monocyte purified from peripheral blood by centtifugal and plastic adhesion technique was cultured with mediators, such as acetyl LDL and autologous platelet, for 5 days under 95% of O_2 and 5% of CO_2. ApoE in the supernatant of cultured media was measured by immunological method using specific anti-apoE antibody. Cholesterol and cholesterol ester in cultured monocyte/macrophage was measured by enzymatic method. In healthy volunteers.secreting apoE and intaking cholesterol in monocyte/macrophage had big differences between individuals. These results suspect that secreting apoE and stored cholesterol might be aspecific and sensitive markersfor suspecting the development of atherosclerosis in the future. The long term follow-up study should be needed to confirm the significance of secreting apoE measurement.We started to study the measuring significance of secreting apoE in clinical status. At first, we evaluate in diavetes mellitus (DM), because DM was very popular disease and we could get much blood from patient's peripheral vessel. We had many troubles in culturing monocyte/macrophage because of contaminating fungus and bacteria. We could not understand what factor affect these troubles. No laboratory markers, such as blood sugar, HbAlc, C-peptide, and/or urinary sugar did not have thedirect relationship tp the trouble, contamination. We kept going the study in DM.If we could, we would like to evaluate the secreting apoE significance in acute myocardial infarction and cerebral infarction, those were typocal clinical of atherosclerosis.