Study of sensory mechanisms from the pelvic viscera

对盆腔内脏感觉机制的研究主要集中在两个方面。用免疫组织化学技术研究了单个靶器官初级感觉神经节神经元的神经递质。P物质、降钙素基因相关肽、亮氨酸脑啡肽、血管活性肠肽在这些神经元中已被发现。VIP主要位于宫颈的传入神经元中。第二个主题是传入终末的激活机制。由于膀胱发生感染，所以称为膀胱炎，出现尿频和疼痛，这可能是由于感染过程中感觉神经末梢的改变。观察炎症过程中白细胞释放的白介素1β(IL1β)的变化。IL1β可降低排尿反射的阈值体积，降低背根记录的放电阈值压力。将膀胱溶液调至较低的PH值(<3.5)可降低C纤维的放电阈值压力。相比之下，Adelta型纤维未见变化。此外，在低pH溶液中，62%的C纤维无反应者发生放电。高pH溶液不改变放电的发生和发生。这些数据表明，膀胱粘膜含有许多肽能递质的盆腔器官初级传入神经元和神经末梢在给药后变得敏感，或将膀胱内溶液改为低pH。因此，C纤维在正常情况下可能是沉默的，在炎症过程中变得活跃，可能是导致病理症状的原因之一。

Study of sensory mechanisms from the pelvic viscera has been focused on two parts. Identify neurotransmitter at primary sensory ganglion neurons was studied for individual target organs using immunohistochemical techniques. Substance P, CGRP, leucine enkephalin, VIP have been identified in these neurons. VIP was highly located in the uterine cervix afferent neurons. Second topic was the mechanisms of activation of the afferent terminals. Since urinary bladder become infection, so call cystitis has been developed with frequent urination and pain, This could be due to change of sensory terminals during the infection. Interleukin 1beta (IL1beta) which released from white blood cells during the inflammation was studied. IL1beta lowered threshold volume of micturition reflex and lowered the threshold pressure for firing at the dorsal root recording. Changing the intravesical solution to lower PH(<3.5) brought the threshold pressure for firing down in C fiber. In contrast no change was observed in Adelta fibers. Additionaly, 62% of C-fiber none responders for disteition became firing after low pH solution. Higher pH solution did not altered the thereshould or occurrence of firing.These data indicated that primary afferent neurons from the pelvic organs containing many peptidergic transmitters and nerve terminals at bladder mucosa become sensitive following administration of Il1beta or changing intravesical solution to lower pH. Thus might be silent C-fiber in normal become active during inflammation and could be contribute the pathological symptom.