Improving the efficiency of CRISPR gene editing in cells

人类红细胞具有良好的特征，珠蛋白基因位点是研究基因调控的模型系统。基因编辑技术和传递工具正在迅速发展，珠蛋白基因座是基因编辑优化的完美模型。新南威尔士大学悉尼分校和CSL之间的合作旨在将我们的专业知识和新技术结合起来，开发一个红细胞基因改造的最佳平台。同时，该项目旨在对人类基因调控机制产生根本性的见解。该项目的技术和生物学成果将有利于未来的基因编辑应用。

Human red blood cells are well-characterised and the globin gene locus is a model system for the study of gene regulation. Gene editing technologies and delivery tools are evolving rapidly and the globin gene locus is the perfect model for gene editing optimisation. This collaboration between UNSW Sydney and CSL aims to bring together our combined expertise and new technologies to develop an optimal platform for genetic modification in a red blood cell line. Simultaneously, this project aims to generate fundamental insights into mechanisms of human gene regulation. The technological and biological outcomes of this project will be of benefit for future gene editing applications.