Potency of calcification in aeticular chondrocyte

本研究的主要目的是如何修复受损的关节面。为此，我们进行了三种实验。首先，将胶原凝胶培养的同基因和同种异体软骨细胞移植到近交系大鼠髌骨沟骨软骨缺损处。术后12个月，组织学观察，免疫组化法检测2型胶原的表达，长期观察表明，同种异体骨移植的骨缺损与同种异体骨移植的骨缺损一样成功愈合，但12周时软骨下骨形成延迟。另一方面，仅植入胶原凝胶或保持开放的对照缺损均未愈合。在第二个实验中，为了研究不同细胞层的软骨细胞在有效钙化方面的差异，我们进行了自体软骨软骨原位移植，并将移植物倒置植入。结果原位移植组几乎完全修复，而倒置移植组关节面被纤维骨组织覆盖。这一结果提示了软骨细胞在不同层次上的特性差异。实验三取髂骨截骨术后10天的骨痂骨组织，植入Raddit股骨髌骨沟骨软骨缺损处，共88只。未成熟骨痂植入的缺损在术后3个月由透明软骨愈合。这些结果为修复关节面骨软骨缺损提供了丰富的信息，并显示了不同软骨细胞层的细胞特性差异。

The main purpose of this study is how to repair damaged joint sunace. For this purpose, we perlormed three kinds of experiments. First, isogenic and allogenic chondrocytes cultured in collagen gel were transplanted into osteo-chondral defects made on patellar groove in inbred strain rats. Till 12 months postperatively, the healing behaviors were histologically observed, including immunohistochemistry to detect type 2 collagen.Long term observation showed that allografted defects healed as successfully as isografted one, although subchondral bone formation was delayd in the foemer at the 12 weeks stage. On the other hand, no control defects which had been implanted with only collagen gel or left open, healed with hyaline carfilage. ln the second experiment, to investigate difference in potent calcification of chondrocytes depending on the cell layr, we performed autograft of arficular carfilage in situ and also implanted up-side-down the graft. As a result, aimost complete repair was obtained in in-situ grafted group, whereas the joint surface were covered by fibro-osseous tissue for the up-side-down grafted group. This result suggests the difference in the character of chondrocytes accoding to the layr. lnthe third experiment, callo-osseous tissue obtained 10 days after iliac osteotomy were implanted to osteo-chondral defects made on patellar groove of raddit's femur, using 88 rabbits. defects where immature callus callus were implanted were healed by hyaline cartilage 3 months postoperativery. These results give us much information, to repair osteochondral defects on joint surface and showed the diffrence of cell character, depending on cartilage cell layr.