Analysis of reproductive processes by means of transgenic animals

本研究旨在通过分析转基因动物的生殖功能来阐明新的生殖调控因素，主要结果如下：1）表达人生长激素的转基因大鼠出现早熟性青春期，表明体重不是诱导青春期的先决条件。2)孕鼠脾内注射巨噬细胞可使着床窗提前打开。3)从携带顶体酶基因靶向突变的小鼠获得的精子可以穿透卵母细胞透明质膜。4)小鼠生殖原细胞的增殖和生长停滞被证明是自主调控的克隆培养。5)在缺乏着色性干皮病A组基因的小鼠中证实了紫外线B或化学致癌物诱导的皮肤肿瘤的高发病率。6)在携带干扰素基因靶向突变的小鼠中发现绒毛膜细胞分化异常。7)结果表明，细胞角蛋白EndoA增强子中的两个Ets结合位点之一是增强子活性所必需的，并与Ets-2相关蛋白结合。8)一个周期性的G蛋白亚基网络与细胞角蛋白丝海星卵母细胞共存。

This Study was performed to elucidate new factors regulating reproductive processes by amalyzing reproductive function in transgenic animals, and following results were obtained.1) Precosious puberty occurred in transgenic rats expressing human growth hormone, suggesting that body weight is not a prerequisite factor for the induction of puberty. 2) By means of administration of macrophages in the spleen of pregnant mice, implantation window could be opened earlier. 3) The sperm obtained from mice carring a targeted mutation of the acrosin gene could penetrate the oocyte zona pellucida. 4) Proliferation and growth arrest in mouse primordal germ cell were shown to be autonomously regulated by means of clonal cultures. 5) High incidence of ultraviolet-B- or chemical-carcinogen-induced skin tumors was demonstrated in mice lacking the xeroderma pigmentosum group A gene. 6) Abnormality in differentiation of chorionic cells was found in mice carring a targeted mutation of the interferon gene. 7) It was revealed that one of two Ets-binding sites in the cytokerarin EndoA enhancer is essential for enhancer activity and bind to Ets-2 related proteins. 8) A periodic network of G protein subunits were shown to coexist with cytokeratin filament starfish oocytes.