Epstein-Barr Virus Infection in Chinese Salivary Cancers

为了确定eb病毒（EBV）在蒙古人涎腺淋巴上皮瘤中的感染情况，我们收集了中国不同地区的涎腺淋巴上皮瘤病例，采用免疫组织化学、原位杂交（ISH）和聚合酶链反应（PCR）技术对其进行了检测。材料与方法：从中国8个城市（长春、北京、西安、武汉、成都、上海、昆明和广州）的9所大学医院收集淋巴上皮瘤和良性淋巴上皮病变患者的唾液组织样本。用福尔马林固定切片和石蜡切片进行上皮细胞和淋巴细胞的免疫组织化学鉴定，用ISH检测EBER-1，用PCR检测EBV DNA的Bam HI - W区。结果与讨论：共收集涎腺淋巴上皮瘤132例（上海41例，成都40例，广州1例，北京10例，武汉10例，长春6例，昆明3例，西安3例），良性淋巴上皮病变57例。成都的高发病率似乎与过去西南沿海的频繁移民有关。淋巴上皮瘤系列患者男66例，女63例（均为汉族，3例，未知），年龄9 ~ 73岁（平均44岁，40%患者年龄小于40岁）。它在年轻一代中的发生是有特点的。超过一半的病例位于腮腺（65%），25%位于小唾液腺。唾液淋巴上皮瘤的癌细胞对EMA、CA153和UEA-I结合呈强免疫阳性，表明其导管上皮性质。它们的细胞质偶有LMP-1免疫阳性。在所有的病例中，细胞核都显示出由ISH引起的强烈的EBER-1信号。间质中以t细胞为主。多数病例的PCR结果均为阳性。而良性淋巴上皮病变未见EBV信号。结果提示EBV在中华唾液淋巴上皮瘤的发病机制中起重要作用，淋巴上皮瘤不是良性淋巴上皮病变的恶性对应物。少

In order to determine prevalence of Epstein-Barr virus (EBV) infection in salivary lymphoepitheliomas which are characaterized by the histology of undifferentiated carcinoma cells with lymphoid stroma and the high incidence among the Mongolid people, we collected cases of this type of carcinomas from different sites of China and examined them by immunohistochemical, insitu hybridization (ISH) and polymerase chain reaction (PCR) techniques.Materials & Methods : Salivary tissue samples from patients with lymphoepitheliomas and benign lymphoepithelial lesions were collected from nine university hospitals at eight cities (Changchun, Beijing, Xi'an, Wuhan, Chengdu, Shanghai, Kunming, and Guangzhou) in China. Formalin-fixed and parafin sections were used for immunohistochemistry for epithelial and lymphocytic characterization, ISH for EBER-1, and PCR for Bam HI W region of EBV DNA.Result & Discussion : We have collected 132 cases of salivary lymphoepitheliomas (41, Shanghai ; 40, Chengdu ; 1 … More 9, Guangzhou ; 10, Beijing ; 10, Wuhan ; 6, Changchun ; 3, Kunming ; 3, Xi'an) and 57 benign lympnoepithelial lesions. The high incidence in Chengdu seems to be related to frequent immigration from the Shouth-West coast in the past. The lymphoepithelioma series consisted of 66 male and 63 female patients (all the Han race ; 3, unknown) , ranging in age from 9 to 73 years (mean, 44 years ; 40% of patients were younger than 40 years) . Its occurrence in the younger generation was charaacteristic. More than half of the cases were located in parotid glands (65%) , and 25% in minor salivary glands. Carcinoma cells of salivary lymphoepitheliomas were strongly immunopositive for EMA, CA153, and UEA-I binding, indicating their duct epithelial natures. Their cytoplasm was occasionaly immunopositive for LMP-1. The nuclei showed intense signals for EBER-1 by ISH in all of the cases examined. T-cells were predominant in the stroma. Positive PCR products were also shown in most of the cases. However, no EBV signals were obtained in the benign lymphoepithelial lesions. The results suggest that EBV play an important role in pathogenesis of the Chinese salivary lymphoepitheliomas, and that lymphoepithelioma is not a malignant counterpart of the benign lymphoepithelial lesion. Less