A novel hair analysis for trace elements on hair cross section by synchrotron radiation X-ray fluorescence imaging with X-ray microprobe.

从1994年到1996年三年的研究结果总结如下：1)在本研究中，我们尝试进行X射线微区分析，即1个像素是5×5微米2，以揭示人体头发横截面的微量元素分布，而不需要清洗样品。本研究使用的SR-XRF成像技术清楚地显示了锌和铅在冶炼厂横断面中的不同分布。2)综述了甲基汞对哺乳动物铜、锌和锰超氧化物歧化酶的影响。通过纯化酶制剂的实验，对体内金属有机化合物选择性降低锰-超氧化物歧化酶的机理进行了广泛的研究。3)血液和头发中的金属水平分别在7天和8.5天达到最大值，但这些消除曲线呈现相似的模式(r=0.97)，但存在时间滞后(约3.5天)。

The result of the studies carried out for three years, 1994 to 1996, can be summarized as follows.1) In this study, we attempted to perform the X-ray microanalysis, ie, one pixel is 5*5mum^2, to reveal trace element distribution of human hair cross sections without washing the samples. The SR-XRF imaging technique used in this study clearly revealed differential distribution of Zn and Pb in the cross hair secton of the smelter.2) A study of alterations in mammalian Cu, Zn- and Mn-superoxide dismutase caused by methylmercury has been reviewed. Mechanisms for the isozyme-elective decrease in Mn-SOD by the organometallic compound observrd in vivo has been extensively examined by experiments with purified enzyme preparations.3) The metal leveals in blood and hair reached a maximum at 7 and 8.5 days, respectively, but these elimination curves exhibited a similar pattern (r=0.97) with a time lag (about 3.5 days).