Basic research for glucose metabolism in inflammatory tissue by positron-labelled glucose analog

1.研究了松节油诱导的炎症组织中18&gt；FDG的组织分布。注射8FDG后1h摄取18FDG达最大值，慢性期摄取高于急性期。2.通过宏观和微观放射自显影(ARG)分析炎症成分因子与18FDG摄取的关系。在宏观和微观ARG上，因摄取18FDG所致的颗粒密度最高的是脓肿壁的边缘地带，其中包括大量中性粒细胞和巨噬细胞的炎症细胞、血管上皮细胞和年轻的成纤维细胞。结果表明，毛细血管通透性增加导致液体渗漏至血管外间隙，以18FDG为能量来源。此外，还研究了血管通透性和血糖的其他因素与FDG摄取的关系。作为血管通透性指标的血清白蛋白和18FDG的双示踪组织分布研究表明，18FDG摄取依赖于血管通透性。在葡萄糖和胰岛素负荷的组织分布研究中，对照组炎症组织对18&gt；FDG的摄取低于葡萄糖负荷组和胰岛素组，表明血糖和胰岛素对炎症组织糖代谢的影响是复杂的。3.在粒细胞集落刺激因子(G-CSF)负荷的组织分布研究中，炎症组织、脾和骨骼的摄取明显高于对照组。这一结果可能反映了G-CSF作为细胞因子的直接作用。4.在双示踪组织分布研究中，在接种松节油后的第4天，两者的摄取量都达到最大值。然而，在双示踪宏观ARG上，最高摄取的是脓肿壁外，最高摄取的是脓肿壁内的18FDG。

1.Tissue distribution of ^<18>FDG in turpentine-induced inflammatory tissue was investigated. Maximum uptake of ^<18>FDG was observed 1 hour after the ^8FDG injection and the uptake in chronic phase was higher than that in acute phase.2.Relations between the inflammatory composition factor and the ^<18>FDG uptake was examined by macro and micro-autoradiography (ARG). On macro-and micro-ARG,the highest grain density due to ^<18>FDG uptake was observed in marginal zone of abscess wall consisting many inflammatory cells of neutrophils and macrophages, epithelial cells of blood vessel and young fibroblasts. Results indicate that increased permeability of capillary blood vessels result in a leakage of fluid including ^<18>FDG into the extra-vascular space and the ^<18>FDG was utilized as energy source. Relations between other factors of vascular permeability and blood glucose and ^<18>FDG uptake were also examined. Double-tracer tissue distribution study, of ^<131>I serum albumin as indicator of vascular permeability and the ^<18>FDG showed that the ^<18>FDG uptake depended on the vascular permeability. In glucose and insulin loaded tissue distribution study, ^<18>FDG uptake in inflammatory tissue in control group was lower than in glucose loaded group and insulin group, thus indicating the complicated influence of blood glucose and insulin on glucose metabolism in inflammtory tissue.3.In granulocyte-colony-stimulating-factor (G-CSF) loaded tissue distribution study, ^<18>FDG uptakes in inflammatory tissue, spleen and bone were significantly higher than those in control group. This result may reflects the direct function of G-CSF as cytokine.4.In double-tracer tissue distribution study of ^<67>Ga citrate and ^<18>FDG,both maximum uptakes were also observed on Day 4 after inoculation of turpentine-oil. However, on double-tracer macro-ARG,the highest uptake of ^<67>Ga citrate was observed outside abscess wall and the highest of ^<18>FDG was inside.