RISK ASSESSMENT OF ENVIRONMENTAL MICROPOLLUTANTS BY USING DNA STRAND BREAKS IN HUMAN CELLS

1)自来水中含氯有机物引起的DNA链断裂日本1993年在自来水水质标准中增加了13种含氯有机物。我们使用FADU方法评估了11种氯化化合物诱导的人类细胞DNA链断裂。我们估计了人成纤维细胞W138-VA13的DNA链断裂。用1，2-二氯乙烷和1，3-二氯丙烯诱导DNA链断裂。2)日本自来水污染物致人细胞DNA链断裂的检测与定量。用Sep-pak C_&lt；18&gt；浓缩自来水样品，用FADU法检测细胞DNA断裂。大阪市自来水样品致人细胞DNA链断裂呈剂量依赖性增加。但这种DNA毒性可通过大鼠肝脏S9组分、谷胱甘肽和水煮来降低。

1) DNA strand breaks induced by chlorinated organo compounds containing in tap waterThirteen chlorinated organo compounds were added to the standard of tap water quality in 1993 in Japan. We assessed the DNA strand breaks in human cells induced by eleven these chlorinated chemicals, using the FADU methods. We estimated DNA strand breaks in human fibroblast cells, W138-VA13. DNA strand breaks were induced by 1,2-dichloroethane and 1,3-dichloropropene.2) Detection and Quantification of DNA Strand Breaks in Human Cells Induced by Contaminants in Japanese Tap Waters.We tried to detect DNA strand breaks caused by tap waters. Tap water samples were concentrated using the Sep-pak C_<18> and DNA strand breaks induced in human cells were measured by FADU assay. The tap water samples of Osaka City induced dose dependent increase in DNA strand breaks in human cells. But this DNA toxicity was reduced by rat liver S9 fraction, glutathione and water boiling.