Evaluation of Brassica crops and wild allies for the breeding by wide cross

本研究通过与野生亲本的宽杂交，选育了芸苔属作物的转基因品系（合成双二倍体品系、外源基因渗入品系、异质品系、单体外源染色体添加品系、单体外源染色体替代品系），取得了以下结果：1)萝卜与4种芸苔属植物新选育了双二倍体系。2)在萝卜和白菜中选育了异质系，部分异质系表现出雄性不育性，可用于杂交制种。3)在萝卜和白菜中培育了外源染色体附加系，并对附加染色体进行了鉴定和评价。4)部分成果已在相应期刊上发表，其他课题已准备发表。5)新开发品系的种子可根据新调查的要求提供，但数量有限。

In this study for the development of genetically modified lines of Brassica crops (Synthetic amphidiploid line, Alien gene (s) introgression line, Alloplasmic line, Monosomic alien chromosome addition line, Monosomic alien chromosome substitution line) by the wide cross with wild allies, following resuls were obtained.1)Amphidiploid lines were newly bred between radish and four Brassica species.2)Alloplasmic lines were bred in radish and Chinese cabbage, some of them exhibiting male steririty effective for hybrid seed production.3)Alien chromosome addition lines were developed in radish and Chinese cabbage in which some added choromosomes were identified and evaluated.4)Some of the resuls were reported in the corresponding journals and the other subject matters are prepared for publication.5)Seeds of newly developed lines may be provided in request for the novel investigation within the limited amount.